Cathepsin L Antibody - #DF12880

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Product: Cathepsin L Antibody
Catalog: DF12880
Description: Rabbit polyclonal antibody to Cathepsin L
Application: WB IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 30 kDa; 38kD(Calculated).
Uniprot: P07711
RRID: AB_2845841

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Cathepsin L Antibody detects endogenous levels of total Cathepsin L.
RRID:
AB_2845841
Cite Format: Affinity Biosciences Cat# DF12880, RRID:AB_2845841.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cathepsin L; cathepsin L, 1 b; Cathepsin L1; Cathepsin L1 light chain; CathepsinL; CATL; CATL1_HUMAN; cb15; CTSL; CTSL1; ctsl1b; FLJ31037; hgg1; Major excreted protein; MEP; MGC123162; wu:fb30g09;

Immunogens

Immunogen:
Uniprot:

P07711(CATL1_HUMAN) >>Visit HPA database.

Gene(ID):
CTSL(1514)
Sequence:
MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV

PTMs - P07711 As Substrate

Site PTM Type Enzyme
K104 Sumoylation
K104 Ubiquitination
K123 Ubiquitination
K216 Ubiquitination
N221 N-Glycosylation
T223 Phosphorylation
K230 Ubiquitination
Y295 Phosphorylation
Y311 Phosphorylation
C322 S-Nitrosylation

Research Backgrounds

Function:

Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity).

Subcellular Location:

Lysosome. Apical cell membrane>Peripheral membrane protein>Extracellular side.
Note: Localizes to the apical membrane of thyroid epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Dimer of a heavy and a light chain linked by disulfide bonds.

Family&Domains:

Belongs to the peptidase C1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

References

1). TFE3 regulates the function of the autophagy-lysosome pathway to drive the invasion and metastasis of papillary thyroid carcinoma. Analytical Cellular Pathology, 2021 (PubMed: 34660181) [IF=3.2]

Application: WB    Species: Human    Sample: PTC cells

Figure 5 TFE3 induces autophagy-lysosome in PTC cells. (a, b) After transfection with pcDNA3.1-TFE3 or si-TFE3, BCPAP and KTC-1 cells showed significant changes in TFE3 protein levels. Overexpression of TFE3 increased the expression of P62/SQSTM1, and the opposite results were observed in BCPAP and KTC-1 cells transfected with si-TFE3. The ratio of LC3II/LC3Iin BCPAP and KTC-1 cells increased significantly after pcDNA3.1-TFE3 transfection, and the opposite results appeared after si-TFE3 transfection. (c) After knocking down TFE3, the expression of LC3 and P62/SQSTM1 in PTC cells was decreased, and overexpression of TFE3 increased the expression of LC3 and P62/SQSTM1 in PTC cells (∗P < 0.05). (d) After knocking down TFE3, the expression of CTSB and CTSL in PTC cells was decreased, while TFE3 is overexpression, the expression of CTSB and CTSL in PTC cells increased (∗P < 0.05).

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