CARNS1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells, using CARNS1 Antibody.

Product:	CARNS1 Antibody
Catalog:	DF12878
Description:	Rabbit polyclonal antibody to CARNS1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Xenopus
Mol.Wt.:	88 kDa; 88kD(Calculated).
Uniprot:	A5YM72
RRID:	AB_2845839

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

CARNS1 Antibody detects endogenous levels of total CARNS1.

RRID:

AB_2845839
Cite Format: Affinity Biosciences Cat# DF12878, RRID:AB_2845839.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP-grasp domain-containing protein 1; Atpgd1; Carnosine synthase 1; Carns1; CRNS1_HUMAN; Kiaa1394;

Immunogens

Immunogen:

Uniprot:

A5YM72(CRNS1_HUMAN) >>Visit HPA database.

Gene(ID):

CARNS1(57571)

Sequence:

A5YM72 CRNS1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLCLSPAWLMKVPAPGQPGEAALLVSKAVSFHPGGLTFLDDFVPPRRATYFLAGLGLGPGRGREAAELARDLTCPTGASAELARLLEDRLLTRQLLAQQGGVAVPATLAFTYKPPGLLRGGDASLGLRLVELSGKEGQETLVKEEVEAFLRSEALGDILQVAVKLSGWRWRGRQAWRLHPRAELGAVVDTVLALLEKLEEEESVLVEAVYPPAQLPCSDGPSPGPGLAVRICAVVCRTQGDRPLLSKVVCGVGRGDRPLRHHNSLPRTLEVALAQCGLGEEAQVAAVRQRVKAAAEAALAAVLALEAGLSAEQRGGRRAHTDFLGVDFALTAAGGVLTPVALELNGGLCLEACGALEGLWAAPRLGPAADEAVAAPLVETMLRRSARCLMEGKQLLVVGAGGVSKKFVWEAARDYGLQLHLVESDPNHFASQLVQTFIHFDMTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAAMRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Xenopus

Chicken

Sheep

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - A5YM72 As Substrate

A5YM72

Site	PTM Type	Source
T239	Phosphorylation	Uniprot
S247	Phosphorylation	Uniprot
Y698	Phosphorylation	Uniprot

Research Backgrounds

A5YM72_CRNS1_HUMAN

Function:

Catalyzes the synthesis of carnosine and homocarnosine. Carnosine is synthesized more efficiently than homocarnosine.

Subunit Structure:

Homotetramer.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Amino acid metabolism > Histidine metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

References

1). Carnosine Synthase 1 Contributes to Interferon Gamma-Induced Arginine Depletion via Mitogen-activated Protein Kinase 11 Signaling in Bovine Mammary Epithelial Cells. Journal of Interferon & Cytokine Research, 2022 (PubMed: 35900262) [IF=2.3]

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For Research Use Only.
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CARNS1 Antibody - #DF12878