BRD2 Antibody - #DF12857

(2)   (4)  
Product: BRD2 Antibody
Catalog: DF12857
Description: Rabbit polyclonal antibody to BRD2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 110 kDa; 88kD(Calculated).
Uniprot: P25440
RRID: AB_2845818

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
BRD2 Antibody detects endogenous levels of total BRD2.
RRID:
AB_2845818
Cite Format: Affinity Biosciences Cat# DF12857, RRID:AB_2845818.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BRD 2; Brd2; BRD2_HUMAN; Bromodomain containing 2; Bromodomain-containing protein 2; D6S113E; DKFZp686N0336; Female sterile homeotic related gene 1; Female sterile homeotic related gene 1, mouse, homolog of; FLJ31942; FSH; FSRG1; KIAA9001; NAT; O27.1.1; Really interesting new gene 3 protein; RING3; RING3 PROTEIN; RNF3;

Immunogens

Immunogen:
Uniprot:

P25440(BRD2_HUMAN) >>Visit HPA database.

Gene(ID):
BRD2(6046)
Sequence:
MLQNVTPHNKLPGEGNAGLLGLGPEAAAPGKRIRKPSLLYEGFESPTMASVPALQLTPANPPPPEVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVTIPKNSHKKGAKLAALQGSVTSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPPLLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTAMPPGLAKSSSESSSEESSSESSSEEEEEEDEEDEEEEESESSDSEEERAHRLAELQEQLRAVHEQLAALSQGPISKPKRKREKKEKKKKRKAEKHRGRAGADEDDKGPRAPRPPQPKKSKKASGSGGGSAALGPSGFGPSGGSGTKLPKKATKTAPPALPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIKKPVGKTKEELALEKKRELEKRLQDVSGQLNSTKKPPKKANEKTESSSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25440 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T6 Phosphorylation
K10 Acetylation
K31 Acetylation
S37 Phosphorylation
Y40 Phosphorylation
S45 Phosphorylation
T47 Phosphorylation
S50 Phosphorylation
T57 Phosphorylation
K118 Ubiquitination
T125 Phosphorylation
S248 Phosphorylation
S253 Phosphorylation
T285 Phosphorylation
T287 Phosphorylation
T291 Phosphorylation
S298 Phosphorylation
S301 Phosphorylation
S305 Phosphorylation
S320 Phosphorylation
S334 Phosphorylation
S340 Phosphorylation
K342 Acetylation
K345 Acetylation
K400 Ubiquitination
R449 Methylation
Y450 Phosphorylation
S538 Phosphorylation
K544 Acetylation
K574 Acetylation
S597 Phosphorylation
S608 Phosphorylation
S611 Phosphorylation
T613 Phosphorylation
K614 Acetylation
K617 Acetylation
T629 Phosphorylation
Y631 Phosphorylation
S633 Phosphorylation
S639 Phosphorylation
S643 Phosphorylation
S651 Phosphorylation
K656 Ubiquitination
K661 Ubiquitination
T690 Phosphorylation
S705 Phosphorylation
Y715 Phosphorylation
T716 Phosphorylation
T724 Phosphorylation
K725 Acetylation
S744 Phosphorylation
S749 Phosphorylation
K760 Acetylation
S775 Phosphorylation
S777 Phosphorylation
S778 Phosphorylation
S779 Phosphorylation
S784 Phosphorylation
S785 Phosphorylation
S786 Phosphorylation
S787 Phosphorylation
S788 Phosphorylation
S789 Phosphorylation
T795 Phosphorylation

Research Backgrounds

Function:

May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.

Subcellular Location:

Nucleus.
Note: Detected on chromatin and nucleosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with E2F1 and with histone H4 acetylated at 'Lys-13'.

(Microbial infection) Interacts with herpes virus 8 protein LANA1.

Family&Domains:

One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.

References

1). Mutation of Basic Residues R283, R286, and K288 in the Matrix Protein of Newcastle Disease Virus Attenuates Viral Replication and Pathogenicity. International Journal of Molecular Sciences, 2023 (PubMed: 36674496) [IF=5.6]
2). TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence, 2020 (PubMed: 32420802) [IF=5.2]

Application: WB    Species: Mouse    Sample: BSR-T7/5 cells

Figure 7. rSS1GFP infection inhibits host cell transcription, RNA processing and modification. (A) The heatmap of representative 20 DEPs related to “Transcription” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (B) The protein-protein interactions of the DEPs related to “Transcription” are analyzed by the STRING software. A red line indicates the presence of fusion evidence; a blue line indicates co-occurrence evidence; a light blue line indicates database evidence; a purple line indicates experimental evidence; a green line indicates neighborhood evidence; a black line indicates co-expression evidence. (C) The heatmap of representative 20 DEPs related to “RNA processing and modification” during rSS1GFP and rSS1GFP-M/NLSm infection at 12 and 24 h. (D) The protein-protein interactions of the DEPs related to “RNA processing and modification” are analyzed by the STRING software. (E) The mRNA expression levels of four selected DEP genes in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were verified by qRT-PCR. (F) The protein expression levels of four DEPs in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm were examined by Western blotting. The relative expression levels of four DEPs were compared with the control GAPDH expression. Error bars represent standard deviations (mean ± SD) (*P < 0.05; **P < 0.01; ***P < 0.001 compared to the value of rSS1GFP-M/NLSm).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.