BHLHE22 Antibody - #DF12854
| Product: | BHLHE22 Antibody |
| Catalog: | DF12854 |
| Description: | Rabbit polyclonal antibody to BHLHE22 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Rabbit, Chicken |
| Mol.Wt.: | 37 kDa; 37kD(Calculated). |
| Uniprot: | Q8NFJ8 |
| RRID: | AB_2845815 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12854, RRID:AB_2845815.
Fold/Unfold
Basic helix loop helix domain containing class B 5 1 2 5; Basic helix loop helix protein 5; Beta3; Beta3a; BHE22_HUMAN; bHLHB5; bHLHe22; CAGL85; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; Class II bHLH protein BHLHB5; TNRC20; Trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein;
Immunogens
- Q8NFJ8 BHE22_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERGMHLGAAAAGEDDLFLHKSLSASTSKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLGCFEPADPEGAGLLLPPPGGGGGGSAGSGGGGGGGVGVPGLLVGSAGVGGDPSLSSLPAGAALCLKYGESASRGSVAESSGGEQSPDDDSDGRCELVLRAGVADPRASPGAGGGGAKAAEGCSNAHLHGGASVPPGGLGGGGGGGSSSGSSGGGGGSGSGSGGSSSSSSSSSKKSKEQKALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQAISAASLPSSAAAAAAAAALHPALGAYEQAAGYPFSAGLPPAASCPEKCALFNSVSSSLCKQCTEKP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NFJ8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot |
Research Backgrounds
Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA (By similarity). In the brain, may function as an area-specific transcription factor that regulates the postmitotic acquisition of area identities and elucidate the genetic hierarchy between progenitors and postmitotic neurons driving neocortical arealization. May be required for the survival of a specific population of inhibitory neurons in the superficial laminae of the spinal chord dorsal horn that may regulate pruritis. Seems to play a crucial role in the retinogenesis, in the specification of amacrine and bipolar subtypes. Forms with PRDM8 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation.
Nucleus.
Brain-specific, with the highest expression in the cerebellum.
Interacts with PRDM8.
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