BCAT2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells, using BCAT2 Antibody.

Product:	BCAT2 Antibody
Catalog:	DF12851
Description:	Rabbit polyclonal antibody to BCAT2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine
Mol.Wt.:	42 kDa; 44kD(Calculated).
Uniprot:	O15382
RRID:	AB_2845812

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(85%)

Clonality:

Polyclonal

Specificity:

BCAT2 Antibody detects endogenous levels of total BCAT2.

RRID:

AB_2845812
Cite Format: Affinity Biosciences Cat# DF12851, RRID:AB_2845812.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BCAM; BCAT 2; BCAT(m); BCAT2; BCAT2_HUMAN; BCATM; BCT2; Branched chain aminotransferase 2, mitochondrial; Branched-chain-amino-acid aminotransferase; ECA40; mitochondrial; Placental protein 18; PP18;

Immunogens

Immunogen:

Uniprot:

O15382(BCAT2_HUMAN) >>Visit HPA database.

Gene(ID):

BCAT2(587)

Expression:

O15382 BCAT2_HUMAN:

Ubiquitous.

Sequence:

O15382 BCAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Horse

Sheep

Xenopus

Pig

Dog

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15382 As Substrate

O15382

Site	PTM Type	Source
K44	Acetylation	Uniprot
K44	Methylation	Uniprot
K73	Acetylation	Uniprot
C135	S-Nitrosylation	Uniprot
K141	Acetylation	Uniprot
Y228	Phosphorylation	Uniprot
K229	Acetylation	Uniprot
K229	Ubiquitination	Uniprot
K246	Acetylation	Uniprot
K246	Ubiquitination	Uniprot
K321	Acetylation	Uniprot
K321	Ubiquitination	Uniprot
C342	S-Nitrosylation	Uniprot
C345	S-Nitrosylation	Uniprot
K353	Acetylation	Uniprot
K353	Ubiquitination	Uniprot
K374	Ubiquitination	Uniprot
K377	Acetylation	Uniprot
K377	Ubiquitination	Uniprot

Research Backgrounds

O15382_BCAT2_HUMAN

Function:

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

Subcellular Location:

Mitochondrion.

Cytoplasm.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Pantothenate and CoA biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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BCAT2 Antibody - #DF12851