Product: BCAT2 Antibody
Catalog: DF12851
Description: Rabbit polyclonal antibody to BCAT2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine
Mol.Wt.: 42 kDa; 44kD(Calculated).
Uniprot: O15382
RRID: AB_2845812

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(85%)
Clonality:
Polyclonal
Specificity:
BCAT2 Antibody detects endogenous levels of total BCAT2.
RRID:
AB_2845812
Cite Format: Affinity Biosciences Cat# DF12851, RRID:AB_2845812.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCAM; BCAT 2; BCAT(m); BCAT2; BCAT2_HUMAN; BCATM; BCT2; Branched chain aminotransferase 2, mitochondrial; Branched-chain-amino-acid aminotransferase; ECA40; mitochondrial; Placental protein 18; PP18;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15382 BCAT2_HUMAN:

Ubiquitous.

Sequence:
MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
85
Horse
77
Sheep
77
Xenopus
77
Pig
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15382 As Substrate

Site PTM Type Enzyme
K44 Acetylation
K44 Methylation
K73 Acetylation
C135 S-Nitrosylation
K141 Acetylation
Y228 Phosphorylation
K229 Acetylation
K229 Ubiquitination
K246 Acetylation
K246 Ubiquitination
K321 Acetylation
K321 Ubiquitination
C342 S-Nitrosylation
C345 S-Nitrosylation
K353 Acetylation
K353 Ubiquitination
K374 Ubiquitination
K377 Acetylation
K377 Ubiquitination

Research Backgrounds

Function:

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

Subcellular Location:

Mitochondrion.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Pantothenate and CoA biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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