BCAT1/ECA39 Antibody - #DF12850

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Product: BCAT1/ECA39 Antibody
Catalog: DF12850
Description: Rabbit polyclonal antibody to BCAT1/ECA39
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: P54687
RRID: AB_2845811

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(80%), Dog(100%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
BCAT1/ECA39 Antibody detects endogenous levels of total BCAT1/ECA39.
RRID:
AB_2845811
Cite Format: Affinity Biosciences Cat# DF12850, RRID:AB_2845811.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCAT; BCAT(c); Bcat1; BCAT1_HUMAN; BCT1; Branched chain amino acid aminotransferase, cytosolic; branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1; branched chain aminotransferase 1, cytosolic; Branched-chain-amino-acid aminotransferase; cytosolic; DKFZp686E12175; EC 2.6.1.42; ECA39; MECA39; placental protein 18; PNAS 121; PP18; Protein ECA39;

Immunogens

Immunogen:
Uniprot:

P54687(BCAT1_HUMAN) >>Visit HPA database.

Gene(ID):
BCAT1(586)
Expression:
P54687 BCAT1_HUMAN:

During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Sequence:
MKDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLTTALEGNRVREMFGSGTACVVCPVSDILYKGETIHIPTMENGPKLASRILSKLTDIQYGREESDWTIVLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Horse
90
Xenopus
90
Chicken
90
Rabbit
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54687 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K2 Ubiquitination
S9 Phosphorylation
T13 Phosphorylation
S18 Phosphorylation
K19 Ubiquitination
K26 Ubiquitination
K28 Ubiquitination
Y90 Phosphorylation
K107 Ubiquitination
R119 Methylation
K134 Acetylation
K134 Ubiquitination
K215 Ubiquitination
K305 Ubiquitination
S331 Phosphorylation
T333 Phosphorylation
S341 Phosphorylation
Y345 Phosphorylation
K346 Ubiquitination
K360 Ubiquitination
K368 Ubiquitination
T370 Phosphorylation

Research Backgrounds

Function:

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Pantothenate and CoA biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

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