Product: B3GNT3 Antibody
Catalog: DF12848
Description: Rabbit polyclonal antibody to B3GNT3
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: Q9Y2A9
RRID: AB_2845809

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(80%), Bovine(80%), Sheep(80%)
Clonality:
Polyclonal
Specificity:
B3GNT3 Antibody detects endogenous levels of total B3GNT3.
RRID:
AB_2845809
Cite Format: Affinity Biosciences Cat# DF12848, RRID:AB_2845809.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B3GAL T8; B3GALT8; B3GN T3; B3GNT 3; Beta 1,3 galactosyltransferase 8; Beta 3 Gal T8; Beta3Gal T8; Beta3GalT8; BGnT 3; BGnT3; Core 1 extending beta 1,3 N acetylglucosaminyltransferase; Core1 beta3GlcNAcT; HP10328; TMEM3; UDP Gal:beta GlcNAc beta 1,3 galactosyltransferase 8; UDP GlcNAc:betaGal beta 1,3 N acetylglucosaminyltransferase 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y2A9 B3GN3_HUMAN:

Expressed in colon, jejunum, stomach, esophagus, placenta and trachea.

Sequence:
MKYLRHRRPNATLILAIGAFTLLLFSLLVSPPTCKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHCRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQRLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Bovine
80
Sheep
80
Dog
70
Xenopus
67
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2A9 As Substrate

Site PTM Type Enzyme
Y120 Phosphorylation
S276 Phosphorylation

Research Backgrounds

Function:

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in colon, jejunum, stomach, esophagus, placenta and trachea.

Family&Domains:

Belongs to the glycosyltransferase 31 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - lacto and neolacto series.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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