Product: AXUD1 Antibody
Catalog: DF12847
Description: Rabbit polyclonal antibody to AXUD1
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 64 kDa; 64kD(Calculated).
Uniprot: Q96S65
RRID: AB_2845808

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AXUD1 Antibody detects endogenous levels of total AXUD1.
RRID:
AB_2845808
Cite Format: Affinity Biosciences Cat# DF12847, RRID:AB_2845808.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4931429D10Rik; Axin 1 Up-regulated; Axin-1 up-regulated gene 1 protein; CSRN1_HUMAN; CSRNP-1; CSRNP1; Cysteine/serine-rich nuclear protein 1; DKFZp566F164; FAM130B; Protein URAX1; TAIP 3; TAIP-3; TGF beta induced apoptosis protein 3; TGF-beta-induced apoptosis protein 3; URAX1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q96S65 CSRN1_HUMAN:

Ubiquitous. Most abundantly expressed in lung, placenta, skeletal muscle, pancreas and leukocyte. Frequently down-regulated in lung, kidney, liver and colon cancers compared with their corresponding normal tissues.

Sequence:
MTGLLKRKFDQLDEDNSSVSSSSSSSGCQSRSCSPSSSVSRAWDSEEEGPWDQMPLPDRDFCGPRSFTPLSILKRARRERPGRVAFDGITVFYFPRCQGFTSVPSRGGCTLGMALRHSACRRFSLAEFAQEQARARHEKLRQRLKEEKLEMLQWKLSAAGVPQAEAGLPPVVDAIDDASVEEDLAVAVAGGRLEEVSFLQPYPARRRRALLRASGVRRIDREEKRELQALRQSREDCGCHCDRICDPETCSCSLAGIKCQMDHTAFPCGCCREGCENPMGRVEFNQARVQTHFIHTLTRLQLEQEAESFRELEAPAQGSPPSPGEEALVPTFPLAKPPMNNELGDNSCSSDMTDSSTASSSASGTSEAPDCPTHPGLPGPGFQPGVDDDSLARILSFSDSDFGGEEEEEEEGSVGNLDNLSCFHPADIFGTSDPGGLASWTHSYSGCSFTSGVLDENANLDASCFLNGGLEGSREGSLPGTSVPPSMDAGRSSSVDLSLSSCDSFELLQALPDYSLGPHYTSQKVSDSLDNIEAPHFPLPGLSPPGDASSCFLESLMGFSEPAAEALDPFIDSQFEDTVPASLMEPVPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
67
Chicken
50
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96S65 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
S30 Phosphorylation
S32 Phosphorylation
S38 Phosphorylation
T68 Phosphorylation
S71 Phosphorylation
K74 Ubiquitination
R106 Methylation
T110 Phosphorylation

Research Backgrounds

Function:

Binds to the consensus sequence 5'-AGAGTG-3' and has transcriptional activator activity (By similarity). May have a tumor-suppressor function. May play a role in apoptosis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Most abundantly expressed in lung, placenta, skeletal muscle, pancreas and leukocyte. Frequently down-regulated in lung, kidney, liver and colon cancers compared with their corresponding normal tissues.

Family&Domains:

Belongs to the AXUD1 family.

References

1). Matrine improves the hepatic microenvironment and reverses epithelial-mesenchymal transition in hepatocellular carcinoma by inhibiting the Wnt-1/β-catenin signaling pathway. American journal of translational research, 2023 (PubMed: 37692966) [IF=2.2]

Application: IHC    Species: Human    Sample: cirrhotic tissues and HCC tissues

Figure 1 Expression of the Wnt-1/β-catenin signaling pathway and EMT-associated proteins in cirrhotic tissues and HCC tissues as assessed by immunohistochemistry. A. Orange signal indicates the presence of Wnt-1, β-catenin, and Axin-1. Wnt-1 and Axin-1 were mainly present in the cytoplasm. β-Catenin was mainly expressed on the cell membrane in cirrhotic tissues and in the cytoplasm and nucleus in HCC tissues (magnification, 400×), P

Application: WB    Species: Human    Sample: cirrhotic tissues and HCC tissues

Figure 1 Expression of the Wnt-1/β-catenin signaling pathway and EMT-associated proteins in cirrhotic tissues and HCC tissues as assessed by immunohistochemistry. A. Orange signal indicates the presence of Wnt-1, β-catenin, and Axin-1. Wnt-1 and Axin-1 were mainly present in the cytoplasm. β-Catenin was mainly expressed on the cell membrane in cirrhotic tissues and in the cytoplasm and nucleus in HCC tissues (magnification, 400×), P

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