ATG2B Antibody - #DF12842
(2)
| Product: | ATG2B Antibody |
| Catalog: | DF12842 |
| Description: | Rabbit polyclonal antibody to ATG2B |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 240 kDa; 233kD(Calculated). |
| Uniprot: | Q96BY7 |
| RRID: | AB_2845803 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
ATG2B Antibody detects endogenous levels of total ATG2B.
RRID:
AB_2845803
Cite Format: Affinity Biosciences Cat# DF12842, RRID:AB_2845803.
Cite Format: Affinity Biosciences Cat# DF12842, RRID:AB_2845803.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
2410024A21Rik; AI047755; AI503411; ATG2 autophagy related 2 homolog B; Atg2b; ATG2B_HUMAN; Autophagy-related protein 2 homolog B; AW558123; C030004M05Rik; C14orf103; C630028L02Rik; FLJ10242; KIAA4067; mKIAA4067;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q96BY7 ATG2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGIVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Sheep
100
Bovine
92
Dog
92
Rabbit
92
Chicken
45
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96BY7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| Y264 | Phosphorylation | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S495 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| Y712 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S735 | Phosphorylation | Uniprot | |
| K819 | Ubiquitination | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| Y876 | Phosphorylation | Uniprot | |
| S886 | Phosphorylation | Uniprot | |
| S899 | Phosphorylation | Uniprot | |
| S1008 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| S1018 | Phosphorylation | Uniprot | |
| T1022 | Phosphorylation | Uniprot | |
| S1179 | Phosphorylation | Uniprot | |
| S1396 | Phosphorylation | Uniprot | |
| Y1524 | Phosphorylation | Uniprot | |
| S1526 | Phosphorylation | Uniprot | |
| K1531 | Ubiquitination | Uniprot | |
| S1572 | Phosphorylation | Uniprot | |
| Y1577 | Phosphorylation | Uniprot | |
| S1579 | Phosphorylation | Uniprot | |
| S1582 | Phosphorylation | Uniprot | |
| S1583 | Phosphorylation | Uniprot | |
| S1585 | Phosphorylation | Uniprot | |
| T1587 | Phosphorylation | Uniprot | |
| Y1664 | Phosphorylation | Uniprot | |
| K1674 | Ubiquitination | Uniprot | |
| S1743 | Phosphorylation | Uniprot | |
| S1751 | Phosphorylation | Uniprot | |
| Y2012 | Phosphorylation | Uniprot | |
| T2014 | Phosphorylation | Uniprot | |
| S2070 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Required for both autophagosome formation and regulation of lipid droplet morphology and dispersion.
Subcellular Location:
Preautophagosomal structure membrane>Peripheral membrane protein. Lipid droplet.
Family&Domains:
Belongs to the ATG2 family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
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