ASIC2 Antibody - #DF12840

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Product: ASIC2 Antibody
Catalog: DF12840
Description: Rabbit polyclonal antibody to ASIC2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Rabbit, Dog, Chicken
Mol.Wt.: 70 kDa; 58kD(Calculated).
Uniprot: Q16515
RRID: AB_2845801

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Rabbit(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
ASIC2 Antibody detects endogenous levels of total ASIC2.
RRID:
AB_2845801
Cite Format: Affinity Biosciences Cat# DF12840, RRID:AB_2845801.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACCN; ACCN1; ACCN1_HUMAN; Acid sensing ion channel 2; Acid-sensing ion channel 2; Amiloride sensitive brain sodium channel; Amiloride sensitive cation channel 1 neuronal (degenerin); Amiloride sensitive cation channel 1 neuronal; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1, neuronal; Amiloride-sensitive cation channel neuronal 1; ASIC2; ASIC2a; BNaC1; BNC1; Brain sodium channel 1; Degenerin; hBNaC1; Mammalian degenerin homolog; MDEG; Neuronal amiloride sensitive cation channel 1;

Immunogens

Immunogen:
Uniprot:

Q16515(ASIC2_HUMAN) >>Visit HPA database.

Gene(ID):
ASIC2(40)
Expression:
Q16515 ASIC2_HUMAN:

Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.

Sequence:
MDLKESPSEGSLQPSSIQIFANTSTLHGIRHIFVYGPLTIRRVLWAVAFVGSLGLLLVESSERVSYYFSYQHVTKVDEVVAQSLVFPAVTLCNLNGFRFSRLTTNDLYHAGELLALLDVNLQIPDPHLADPSVLEALRQKANFKHYKPKQFSMLEFLHRVGHDLKDMMLYCKFKGQECGHQDFTTVFTKYGKCYMFNSGEDGKPLLTTVKGGTGNGLEIMLDIQQDEYLPIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLTYLPPPWGECRSSEMGLDFFPVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLLAEKDSNYCLCRTPCNLTRYNKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQKKAYEVAALLGDIGGQMGLFIGASILTILELFDYIYELIKEKLLDLLGKEEDEGSHDENVSTCDTMPNHSETISHTVNVPLQTTLGTLEEIAC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Chicken
89
Zebrafish
67
Pig
0
Horse
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16515 As Substrate

Site PTM Type Enzyme
T39 Phosphorylation
Y357 Phosphorylation

Research Backgrounds

Function:

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.

Subunit Structure:

Homotrimer or heterotrimer with other ASIC proteins (By similarity). Interacts with STOM; this regulates channel activity (By similarity). Interacts with PRKCABP and ASIC3. Heterotrimer of Asic1a-Asic2a interacts with the snake venom mambalgin-1. Heterotrimer of Asic1a-Asic2a interacts with the snake venom mambalgin-2 and mambalgin-3 (By similarity). Heterotrimer of Asic1a-Asic2b interacts with the snake venom mambalgin-1 and mambalgin-2 (By similarity).

Family&Domains:

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC2 subfamily.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

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