ARP2 Antibody - #DF12837

(3)  
Product: ARP2 Antibody
Catalog: DF12837
Description: Rabbit polyclonal antibody to ARP2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 45 kDa; 45kD(Calculated).
Uniprot: P61160
RRID: AB_2845798

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ARP2 Antibody detects endogenous levels of total ARP2.
RRID:
AB_2845798
Cite Format: Affinity Biosciences Cat# DF12837, RRID:AB_2845798.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin like protein 2; Actin related protein 2; Actin-like protein 2; Actin-related protein 2; ACTR2; ARP2 actin related protein 2 homolog (yeast); ARP2; ARP2/3 COMPLEX; ARP2_HUMAN; OTTHUMP00000159937; OTTHUMP00000202270;

Immunogens

Immunogen:
Uniprot:

P61160(ARP2_HUMAN) >>Visit HPA database.

Gene(ID):
ACTR2(10097)
Sequence:
MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61160 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S3 Phosphorylation
K7 Ubiquitination
K19 Methylation
K19 Ubiquitination
Y22 Phosphorylation
T45 Phosphorylation
K46 Ubiquitination
K53 Ubiquitination
S62 Phosphorylation
Y72 Phosphorylation
K86 Ubiquitination
Y91 Phosphorylation
T92 Phosphorylation
K97 Ubiquitination
K106 Ubiquitination
T117 Phosphorylation
K118 Ubiquitination
K195 Ubiquitination
Y202 Phosphorylation Q99558 (MAP3K14)
S207 Phosphorylation
K219 Ubiquitination
T237 Phosphorylation Q99558 (MAP3K14)
T238 Phosphorylation Q99558 (MAP3K14)
K253 Ubiquitination
K299 Acetylation
K299 Ubiquitination
K322 Acetylation
K322 Ubiquitination
K331 Sumoylation
K331 Ubiquitination
K336 Ubiquitination
K368 Ubiquitination
T374 Phosphorylation
Y378 Phosphorylation
K381 Ubiquitination
K388 Sumoylation
K388 Ubiquitination

Research Backgrounds

Function:

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

Family&Domains:

Belongs to the actin family. ARP2 subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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