Product: ALG1 Antibody
Catalog: DF12821
Description: Rabbit polyclonal antibody to ALG1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Dog, Chicken
Mol.Wt.: 53 kDa; 53kD(Calculated).
Uniprot: Q9BT22
RRID: AB_2845782

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(89%), Dog(89%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
ALG1 Antibody detects endogenous levels of total ALG1.
RRID:
AB_2845782
Cite Format: Affinity Biosciences Cat# DF12821, RRID:AB_2845782.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4-mannosyltransferase; Alg1; ALG1_HUMAN; asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase); asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae); Asparagine-linked glycosylation protein 1 homolog; Beta 1 4 mannosyltransferase; Beta-1; CDG1K; Chitobiosyldiphosphodolichol beta-mannosyltransferase; GDP Man GlcNAc2 PP dolichol mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; Hmat-1; HMAT1; HMT 1; HMT1; Mannosyltransferase 1; Mannosyltransferase-1; MT 1; MT-1; MT1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVQTVLPLVMDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
89
Dog
89
Chicken
89
Xenopus
78
Zebrafish
56
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BT22 As Substrate

Site PTM Type Enzyme
S46 Phosphorylation
K183 Ubiquitination
K217 Ubiquitination
K223 Ubiquitination
K236 Ubiquitination
S239 Phosphorylation
S242 Phosphorylation
K322 Ubiquitination

Research Backgrounds

Function:

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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