AKR7A3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells, using AKR7A3 Antibody.

Product:	AKR7A3 Antibody
Catalog:	DF12819
Description:	Rabbit polyclonal antibody to AKR7A3
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	37 kDa; 37kD(Calculated).
Uniprot:	O95154
RRID:	AB_2845780

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

AKR7A3 Antibody detects endogenous levels of total AKR7A3.

RRID:

AB_2845780
Cite Format: Affinity Biosciences Cat# DF12819, RRID:AB_2845780.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AFAR 2; AFAR2; AFB1 aldehyde reductase 2; AFB1 AR 2; AFB1-AR 2; Aflatoxin aldehyde reductase; Aflatoxin B1 aldehyde reductase 2; Aflatoxin B1 aldehyde reductase member 3; Akr7a3; Aldo keto reductase family 7 member A3; ARK73_HUMAN; OTTHUMP00000002623;

Immunogens

Immunogen:

Uniprot:

O95154(ARK73_HUMAN) >>Visit HPA database.

Gene(ID):

AKR7A3(22977)

Expression:

O95154 ARK73_HUMAN:

Expressed in colon, kidney, liver, pancreas, adenocarcinoma and endometrium.

Sequence:

O95154 ARK73_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRQLSRARPATVLGAMEMGRRMDAPTSAAVTRAFLERGHTEIDTAFVYSEGQSETILGGLGLRLGGSDCRVKIDTKAIPLFGNSLKPDSLRFQLETSLKRLQCPRVDLFYLHMPDHSTPVEETLRACHQLHQEGKFVELGLSNYAAWEVAEICTLCKSNGWILPTVYQGMYNAITRQVETELFPCLRHFGLRFYAFNPLAGGLLTGKYKYEDKNGKQPVGRFFGNTWAEMYRNRYWKEHHFEGIALVEKALQAAYGASAPSMTSATLRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAAAEEGPLEPAVVDAFNQAWHLVTHECPNYFR

PTMs - O95154 As Substrate

O95154

Site	PTM Type	Source
S6	Phosphorylation	Uniprot
T12	Phosphorylation	Uniprot
R21	Methylation	Uniprot
T27	Phosphorylation	Uniprot
S28	Phosphorylation	Uniprot
T32	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
S90	Phosphorylation	Uniprot
Y111	Phosphorylation	Uniprot
S118	Phosphorylation	Uniprot
S143	Phosphorylation	Uniprot
T227	Phosphorylation	Uniprot
S259	Phosphorylation	Uniprot

Research Backgrounds

O95154_ARK73_HUMAN

Function:

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Expressed in colon, kidney, liver, pancreas, adenocarcinoma and endometrium.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

Research Fields

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

Restrictive clause

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For Research Use Only.
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AKR7A3 Antibody - #DF12819