AKAP8L Antibody - #DF12818

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Product: AKAP8L Antibody
Catalog: DF12818
Description: Rabbit polyclonal antibody to AKAP8L
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 72 kDa; 72kD(Calculated).
Uniprot: Q9ULX6
RRID: AB_2845779

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AKAP8L Antibody detects endogenous levels of total AKAP8L.
RRID:
AB_2845779
Cite Format: Affinity Biosciences Cat# DF12818, RRID:AB_2845779.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein 8 like; A kinase anchor protein 8 like; AKAP 8L; AKAP8 like protein; DKFZp434L0650; HA95; HAP95; Helicase A binding protein 95; Homologous to AKAP95 protein; NAKAP; NAKAP95; Neighbor of A kinase anchoring protein 95; Neighbor of AKAP95;

Immunogens

Immunogen:
Uniprot:

Q9ULX6(AKP8L_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP8L(26993)
Expression:
Q9ULX6 AKP8L_HUMAN:

Ubiquitously expressed. Expressed in the brain cortex (at protein level).

Sequence:
MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEGYGYGYGYGQDNTTNYGYGMATSHSWEMPSSDTNANTSASGSASADSVLSRINQRLDMVPHLETDMMQGGVYGSGGERYDSYESCDSRAVLSERDLYRSGYDYSELDPEMEMAYEGQYDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMWEDPMGARGQCMSGASRLPSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADFRTKKKKRKQGGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEEGKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTADFLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVEAAHCAACDLFIPMQFGIIQKHLKTMDHNRNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTDSPEEEKEQEEAEGGALDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGGALQRQIRGIPGLDVEDDEEGGGGAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULX6 As Substrate

Site PTM Type Enzyme
Y29 Phosphorylation
Y31 Phosphorylation
S117 Phosphorylation
Y125 Phosphorylation
C128 S-Nitrosylation
R131 Methylation
S135 Phosphorylation
R171 Methylation
R179 Methylation
R185 Methylation
R188 Methylation
R191 Methylation
Y197 Phosphorylation
R199 Methylation
R208 Methylation
R217 Methylation
R237 Methylation
R247 Methylation
K257 Acetylation
K257 Ubiquitination
K264 Ubiquitination
T267 Phosphorylation
T268 Phosphorylation
R272 Methylation
S283 Phosphorylation
S289 Phosphorylation
K290 Ubiquitination
T292 Phosphorylation
T294 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S302 Phosphorylation
T308 Phosphorylation
T313 Phosphorylation
S326 Phosphorylation
K362 Ubiquitination
R397 Methylation
K411 Ubiquitination
K414 Ubiquitination
K418 Ubiquitination
K423 Ubiquitination
K426 Ubiquitination
Y435 Phosphorylation
K439 Ubiquitination
K448 Ubiquitination
K477 Ubiquitination
K500 Acetylation
K503 Ubiquitination
T504 Phosphorylation
K520 Acetylation
K533 Ubiquitination
K544 Sumoylation
K544 Ubiquitination
T550 Phosphorylation
S552 Phosphorylation
S601 Phosphorylation

Research Backgrounds

Function:

Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8. May be involved in regulation of pre-mRNA splicing.

(Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters.

(Microbial infection) Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses.

(Microbial infection) In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity.

PTMs:

Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.

Subcellular Location:

Nucleus. Nucleus matrix. Nucleus speckle. Nucleus>PML body. Cytoplasm.
Note: Colocalizes with PRPF40A in the nuclear matrix (PubMed:16391387). Nuclear at steady state but shuttles between the nucleus and cytoplasm (PubMed:10748171). The shuttling property has been questioned (PubMed:11034899). Colocalizes with EBNA-LP in PML bodies (PubMed:11884601).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed in the brain cortex (at protein level).

Subunit Structure:

Interacts (via N-terminus) with DHX9 (via RGG region). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B. Interacts with HDAC3; increased during mitosis. Interacts with EBV EBNA-LP. Interacts with HIV-1 reverse transcriptase/ribonuclease H.

Family&Domains:

Belongs to the AKAP95 family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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