AFAP1L2 Antibody - #DF12813

(3)  
Product: AFAP1L2 Antibody
Catalog: DF12813
Description: Rabbit polyclonal antibody to AFAP1L2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 130 kDa; 91kD(Calculated).
Uniprot: Q8N4X5
RRID: AB_2845774

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Rabbit(92%), Dog(92%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
AFAP1L2 Antibody detects endogenous levels of total AFAP1L2.
RRID:
AB_2845774
Cite Format: Affinity Biosciences Cat# DF12813, RRID:AB_2845774.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin filament-associated protein 1-like 2; AF1L2_HUMAN; AFAP1-like protein 2; afap1l2; CTB-1144G6.4; KIAA1914; XB130;

Immunogens

Immunogen:
Uniprot:

Q8N4X5(AF1L2_HUMAN) >>Visit HPA database.

Gene(ID):
AFAP1L2(84632)
Expression:
Q8N4X5 AF1L2_HUMAN:

Detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas.

Sequence:
MERYKALEQLLTELDDFLKILDQENLSSTALVKKSCLAELLRLYTKSSSSDEEYIYMNKVTINKQQNAESQGKAPEEQGLLPNGEPSQHSSAPQKSLPDLPPPKMIPERKQLAIPKTESPEGYYEEAEPYDTSLNEDGEAVSSSYESYDEEDGSKGKSAPYQWPSPEAGIELMRDARICAFLWRKKWLGQWAKQLCVIKDNRLLCYKSSKDHSPQLDVNLLGSSVIHKEKQVRKKEHKLKITPMNADVIVLGLQSKDQAEQWLRVIQEVSGLPSEGASEGNQYTPDAQRFNCQKPDIAEKYLSASEYGSSVDGHPEVPETKDVKKKCSAGLKLSNLMNLGRKKSTSLEPVERSLETSSYLNVLVNSQWKSRWCSVRDNHLHFYQDRNRSKVAQQPLSLVGCEVVPDPSPDHLYSFRILHKGEELAKLEAKSSEEMGHWLGLLLSESGSKTDPEEFTYDYVDADRVSCIVSAAKNSLLLMQRKFSEPNTYIDGLPSQDRQEELYDDVDLSELTAAVEPTEEATPVADDPNERESDRVYLDLTPVKSFLHGPSSAQAQASSPTLSCLDNATEALPADSGPGPTPDEPCIKCPENLGEQQLESLEPEDPSLRITTVKIQTEQQRISFPPSCPDAVVATPPGASPPVKDRLRVTSAEIKLGKNRTEAEVKRYTEEKERLEKKKEEIRGHLAQLRKEKRELKETLLKCTDKEVLASLEQKLKEIDEECRGEESRRVDLELSIMEVKDNLKKAEAGPVTLGTTVDTTHLENVSPRPKAVTPASAPDCTPVNSATTLKNRPLSVVVTGKGTVLQKAKEWEKKGAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Dog
92
Rabbit
92
Chicken
82
Xenopus
75
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N4X5 As Substrate

Site PTM Type Enzyme
S50 Phosphorylation
Y54 Phosphorylation P07949 (RET)
Y56 Phosphorylation
T61 Phosphorylation
S119 Phosphorylation
Y124 Phosphorylation
Y161 Phosphorylation
S165 Phosphorylation
S213 Phosphorylation
T284 Phosphorylation
Y301 Phosphorylation
S303 Phosphorylation
Y307 Phosphorylation
K332 Methylation
S334 Phosphorylation
S344 Phosphorylation
T345 Phosphorylation
S346 Phosphorylation
S358 Phosphorylation
Y359 Phosphorylation
Y383 Phosphorylation
S397 Phosphorylation
S408 Phosphorylation
Y413 Phosphorylation
S414 Phosphorylation
S431 Phosphorylation
S432 Phosphorylation
T456 Phosphorylation
Y457 Phosphorylation
Y459 Phosphorylation
S466 Phosphorylation
S484 Phosphorylation
Y489 Phosphorylation
Y537 Phosphorylation
T541 Phosphorylation
S600 Phosphorylation
T635 Phosphorylation
S640 Phosphorylation
S651 Phosphorylation
Y668 Phosphorylation
T669 Phosphorylation
K697 Acetylation
S767 Phosphorylation
T774 Phosphorylation
T788 Phosphorylation
S796 Phosphorylation

Research Backgrounds

Function:

May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation.

PTMs:

Tyrosine phosphorylated (by SRC).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas.

Subunit Structure:

Interacts with SRC. Interacts with LCK when tyrosine phosphorylated.

Restrictive clause

 

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