AFAP1 Antibody - #DF12812

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Product: AFAP1 Antibody
Catalog: DF12812
Description: Rabbit polyclonal antibody to AFAP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 110 kDa; 81kD(Calculated).
Uniprot: Q8N556
RRID: AB_2845773

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
AFAP1 Antibody detects endogenous levels of total AFAP1.
RRID:
AB_2845773
Cite Format: Affinity Biosciences Cat# DF12812, RRID:AB_2845773.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

110 kDa actin filament associated protein; 110 kDa actin filament-associated protein; Actin filament associated protein 1; Actin filament associated protein 110 kDa; Actin filament-associated protein 1; AFAP 1; AFAP 110; AFAP; AFAP-110; Afap1; AFAP1_HUMAN; AFAP110; FLJ56849;

Immunogens

Immunogen:
Uniprot:

Q8N556(AFAP1_HUMAN) >>Visit HPA database.

Gene(ID):
AFAP1(60312)
Expression:
Q8N556 AFAP1_HUMAN:

Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.

Sequence:
MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKLSSERPSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCRGHVLRKAKEWELKNGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
88
Chicken
88
Pig
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N556 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y19 Phosphorylation
K42 Ubiquitination
K47 Ubiquitination
Y93 Phosphorylation P12931 (SRC)
Y94 Phosphorylation P12931 (SRC)
S101 Phosphorylation
Y125 Phosphorylation P12931 (SRC)
K208 Acetylation
K212 Acetylation
K234 Ubiquitination
K242 Ubiquitination
Y248 Phosphorylation
S257 Phosphorylation
S264 Phosphorylation
S265 Phosphorylation
S277 Phosphorylation P17252 (PRKCA)
S278 Phosphorylation
S282 Phosphorylation
S283 Phosphorylation
T315 Phosphorylation
S329 Phosphorylation
S336 Phosphorylation
T337 Phosphorylation
T341 Phosphorylation
S342 Phosphorylation
S343 Phosphorylation
T350 Phosphorylation
Y353 Phosphorylation
K373 Ubiquitination
K378 Ubiquitination
K384 Ubiquitination
Y451 Phosphorylation P12931 (SRC)
Y453 Phosphorylation P12931 (SRC)
Y483 Phosphorylation
T487 Phosphorylation
S488 Phosphorylation
Y491 Phosphorylation
S495 Phosphorylation
T497 Phosphorylation
Y501 Phosphorylation
S510 Phosphorylation
S520 Phosphorylation
T524 Phosphorylation
K528 Ubiquitination
T546 Phosphorylation
S548 Phosphorylation
Y553 Phosphorylation
K554 Ubiquitination
K570 Ubiquitination
K606 Ubiquitination
K614 Ubiquitination
S631 Phosphorylation
T637 Phosphorylation
S661 Phosphorylation
T663 Phosphorylation
S664 Phosphorylation
S665 Phosphorylation
S668 Phosphorylation
T675 Phosphorylation
S679 Phosphorylation
S682 Phosphorylation
S683 Phosphorylation
T686 Phosphorylation
S687 Phosphorylation
T689 Phosphorylation
S710 Phosphorylation
S711 Phosphorylation
T730 Phosphorylation

Research Backgrounds

Function:

Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.

PTMs:

Phosphorylated on tyrosine residues by SRC.

Subcellular Location:

Cytoplasm>Cytoskeleton>Stress fiber.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.

Subunit Structure:

Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers (By similarity). Interacts with activated SRC SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB and PRKCI (By similarity).

Restrictive clause

 

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