ADIPOR2 Antibody | Affinity Biosciences

WB
IHC
Cites

1/4

Western blot analysis of extracts from HeLa cells, using ADIPOR2 Antibody.

DF12811 at 1/100 staining rat muscle tissue by IHC-P.

Figure 5 ATL III treatment restores the down-regulation of hepatic AdipoR1 expression and AdipoR1 signaling induced by HFD feeding in mice.

Figure 5 Effects of the water extract of fermented rice bran (FRB) on hepatic lipid metabolism-related protein expressions in aged rats with high-fat (HF) diet feeding.

Product:	ADIPOR2 Antibody
Catalog:	DF12811
Description:	Rabbit polyclonal antibody to ADIPOR2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Dog
Mol.Wt.:	50 kDa; 44kD(Calculated).
Uniprot:	Q86V24
RRID:	AB_2845772

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(83%), Sheep(83%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ADIPOR2 Antibody detects endogenous levels of total ADIPOR2.

RRID:

AB_2845772
Cite Format: Affinity Biosciences Cat# DF12811, RRID:AB_2845772.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

;PAQR2; ACDCR2; Adiponectin receptor protein 2; Adipor2; ADR2_HUMAN; Progestin and adipoQ receptor family member II;

Immunogens

Immunogen:

Uniprot:

Q86V24(PAQR2_HUMAN) >>Visit HPA database.

Gene(ID):

ADIPOR2(79602)

Expression:

Q86V24 PAQR2_HUMAN:

Ubiquitous (PubMed:16044242). Highly expressed in skeletal muscle, liver and placenta (PubMed:12802337). Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung (PubMed:12802337).

Sequence:

Q86V24 PAQR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNEPTENRLGCSRTPEPDIRLRKGHQLDGTRRGDNDSHQGDLEPILEASVLSSHHKKSSEEHEYSDEAPQEDEGFMGMSPLLQAHHAMEKMEEFVCKVWEGRWRVIPHDVLPDWLKDNDFLLHGHRPPMPSFRACFKSIFRIHTETGNIWTHLLGCVFFLCLGIFYMFRPNISFVAPLQEKVVFGLFFLGAILCLSFSWLFHTVYCHSEGVSRLFSKLDYSGIALLIMGSFVPWLYYSFYCNPQPCFIYLIVICVLGIAAIIVSQWDMFATPQYRGVRAGVFLGLGLSGIIPTLHYVISEGFLKAATIGQIGWLMLMASLYITGAALYAARIPERFFPGKCDIWFHSHQLFHIFVVAGAFVHFHGVSNLQEFRFMIGGGCSEEDAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Bovine

Sheep

Chicken

Xenopus

Pig

Horse

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86V24 As Substrate

Q86V24

Site	PTM Type	Source
K23	Ubiquitination	Uniprot
K56	Ubiquitination	Uniprot
S65	Phosphorylation	Uniprot
K90	Ubiquitination	Uniprot
K97	Ubiquitination	Uniprot
K116	Ubiquitination	Uniprot
K137	Ubiquitination	Uniprot

Research Backgrounds

Q86V24_PAQR2_HUMAN

Function:

Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localized to the cell membrane and intracellular organelles.

Tissue Specificity:

Ubiquitous. Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.

Subunit Structure:

May form homooligomers and heterooligomers with ADIPOR1. Interacts with APPL2 (via BAR domain); ADIPOQ dissociates this interaction (By similarity).

Family&Domains:

The N-terminus is cytoplasmic and the C-terminus is extracellular, contrary to what is observed for G-protein coupled receptors. Unlike G-protein coupled receptors, transmembrane helices are not kinked or tilted relative to the plane of the membrane.

Belongs to the ADIPOR family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). Atractylenolide III ameliorates Non-Alcoholic Fatty Liver Disease by activating Hepatic Adiponectin Receptor 1-Mediated AMPK Pathway. International Journal of Biological Sciences (PubMed: 35280674) [IF=9.2]

Application: WB Species: Mouse Sample:

Figure 5 ATL III treatment restores the down-regulation of hepatic AdipoR1 expression and AdipoR1 signaling induced by HFD feeding in mice. A. Expression levels of hepatic AdipoR1 and AdipoR2 were examined in HFD mice. B. Relative levels of hepatic AdipoR1 and AdipoR2 were analyzed. C. AdipoR1 downstream signaling molecules including p-LKB1, p-AMPK, SIRT1, Nrf2, SIRT3, CPT1A and PGC1α were tested in HFD mice. D. Relative levels of p-LKB1, p-AMPK, SIRT1, Nrf2, SIRT3, CPT1A and PGC1α were analyzed.

2). Effects of the Water Extract of Fermented Rice Bran on Liver Damage and Intestinal Injury in Aged Rats with High-Fat Diet Feeding. Plants (PubMed: 35270077) [IF=4.5]

Application: WB Species: Rat Sample:

Figure 5 Effects of the water extract of fermented rice bran (FRB) on hepatic lipid metabolism-related protein expressions in aged rats with high-fat (HF) diet feeding. Values are presented as the mean ± standard deviation (n = 6). * p < 0.05 vs. the normal control (NC) group; significance between two groups was determined using Student’s t-test. In the HF diet-fed groups, significant differences between groups were determined by a one-way ANOVA with Fisher‘s post hoc test. Western blot analysis of (A) NAD-dependent sirtuin-1 (SIRT1), (B) adenosine monophosphate-activated protein kinase-α (AMPKα), (C) phosphorylated (p)-AMPKα, (D) p-AMPKα/AMPKα, (E) leptin receptor, and (F) adiponectin receptor 2 (AdipoR2) protein expressions. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) was used as an internal control. Quantitative analysis of protein levels and the ratio of each internal control were calculated by setting the value of the mean of the NC group.

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ADIPOR2 Antibody - #DF12811