XAB2 Antibody - #DF12798

(3)  
Product: XAB2 Antibody
Catalog: DF12798
Description: Rabbit polyclonal antibody to XAB2
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 100 kDa; 100kD(Calculated).
Uniprot: Q9HCS7
RRID: AB_2845759

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
XAB2 Antibody detects endogenous levels of total XAB2.
RRID:
AB_2845759
Cite Format: Affinity Biosciences Cat# DF12798, RRID:AB_2845759.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

0610041O14Rik; adapter protein ATH 55; Adapter protein ATH-55; Ath55; AV025587; Crn related protein kim1; crn-related protein kim1; DKFZp762C1015; HCNP; HCNP protein; HCRN; KIAA1177; NTC90; PP3898; Pre-mRNA-splicing factor SYF1; Protein HCNP; RNA splicing factor; SYF1; SYF1 homolog; SYF1 homolog, RNA splicing factor; SYF1 pre mRNA splicing factor; SYF1_HUMAN; Xab2; XPA binding protein 2; XPA-binding protein 2;

Immunogens

Immunogen:
Uniprot:

Q9HCS7(SYF1_HUMAN) >>Visit HPA database.

Gene(ID):
XAB2(56949)
Sequence:
MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERALKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFLMDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESAEEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNVDAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYAQFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEWHKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEKATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRVYKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKAYERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYAQLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLSDEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTIKEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLAAEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLEQQSVPAAVFGSLKED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Xenopus
92
Zebrafish
92
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HCS7 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
K36 Ubiquitination
K45 Ubiquitination
K62 Ubiquitination
C66 S-Nitrosylation
K69 Ubiquitination
K174 Ubiquitination
Y183 Phosphorylation
K188 Ubiquitination
K212 Ubiquitination
K215 Ubiquitination
Y279 Phosphorylation
S315 Phosphorylation
K389 Ubiquitination
K393 Ubiquitination
K420 Acetylation
K420 Ubiquitination
K423 Ubiquitination
K458 Ubiquitination
Y469 Phosphorylation
R479 Methylation
K482 Ubiquitination
S496 Phosphorylation
T499 Phosphorylation
K539 Ubiquitination
K590 Ubiquitination
T620 Phosphorylation
K637 Ubiquitination
K654 Ubiquitination
K677 Ubiquitination
T706 Phosphorylation
K708 Ubiquitination
K721 Ubiquitination
Y734 Phosphorylation
K770 Ubiquitination
K794 Ubiquitination
S851 Phosphorylation
K853 Ubiquitination

Research Backgrounds

Function:

Involved in pre-mRNA splicing as component of the spliceosome. Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing.

Subcellular Location:

Nucleus.
Note: Detected in the splicing complex carrying pre-mRNA.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associates with RNA polymerase II, the TCR-specific proteins CKN1/CSA and ERCC6/CSB, and XPA. Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19.

Family&Domains:

Belongs to the crooked-neck family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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