VPS45 Antibody - #DF12796

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Product: VPS45 Antibody
Catalog: DF12796
Description: Rabbit polyclonal antibody to VPS45
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: Q9NRW7
RRID: AB_2845757

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
VPS45 Antibody detects endogenous levels of total VPS45.
RRID:
AB_2845757
Cite Format: Affinity Biosciences Cat# DF12796, RRID:AB_2845757.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q9NRW7(VPS45_HUMAN) >>Visit HPA database.

Gene(ID):
VPS45(11311)
Expression:
Q9NRW7 VPS45_HUMAN:

Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.

Sequence:
MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
92
Zebrafish
82
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRW7 As Substrate

Site PTM Type Enzyme
Y10 Phosphorylation
K76 Ubiquitination
Y81 Phosphorylation
S168 Phosphorylation
K185 Ubiquitination
K192 Ubiquitination
K197 Ubiquitination
K257 Ubiquitination
Y272 Phosphorylation
K296 Ubiquitination
K304 Ubiquitination
S307 Phosphorylation
K312 Ubiquitination
Y318 Phosphorylation
K322 Methylation
K322 Ubiquitination
K323 Methylation
T327 Phosphorylation
K330 Ubiquitination
S339 Phosphorylation
K371 Ubiquitination
K378 Ubiquitination
K418 Ubiquitination
S424 Phosphorylation
K432 Ubiquitination
S437 Phosphorylation
S441 Phosphorylation
K443 Ubiquitination
K450 Ubiquitination
K454 Ubiquitination
K457 Ubiquitination
K479 Ubiquitination
K483 Ubiquitination
Y487 Phosphorylation
K557 Ubiquitination
S559 Phosphorylation
S560 Phosphorylation
T563 Phosphorylation
S564 Phosphorylation

Research Backgrounds

Function:

May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein.
Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver.

Subunit Structure:

Interacts with STX6 (By similarity). Interacts with ZFYVE20.

Family&Domains:

Belongs to the STXBP/unc-18/SEC1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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