UMPS Antibody | Affinity Biosciences

WB
IF/ICC

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Western blot analysis of extracts from Jurkat cells, using UMPS Antibody.

Western blot analysis of extracts from Hela cells, using UMPS Antibody.

Product:	UMPS Antibody
Catalog:	DF12790
Description:	Rabbit polyclonal antibody to UMPS
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.:	52 kDa; 52kD(Calculated).
Uniprot:	P11172
RRID:	AB_2845751

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(90%), Horse(90%), Rabbit(90%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

UMPS Antibody detects endogenous levels of total UMPS.

RRID:

AB_2845751
Cite Format: Affinity Biosciences Cat# DF12790, RRID:AB_2845751.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ODC; OMPdecase; OPRT; OPRTase; Orotate phosphoribosyl transferase and orotidine 5' decarboxylase; Orotate phosphoribosyltransferase; Orotate phosphoribosyltransferase phosphoribosyltransferase; Orotidine 5' phosphate decarboxylase; Orotidine 5''-phosphate decarboxylase; RP11-71H17.9; UMP synthase; Umps; UMPS_HUMAN; Uridine 5' monophosphate synthase; Uridine monophosphate synthetase;

Immunogens

Immunogen:

Uniprot:

P11172(UMPS_HUMAN) >>Visit HPA database.

Gene(ID):

UMPS(7372)

Sequence:

P11172 UMPS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Horse

Bovine

Rabbit

Chicken

Xenopus

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11172 As Substrate

P11172

Site	PTM Type	Source
A2	Acetylation	Uniprot
Y16	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
K29	Ubiquitination	Uniprot
S34	Phosphorylation	Uniprot
Y37	Phosphorylation	Uniprot
K106	Acetylation	Uniprot
K145	Ubiquitination	Uniprot
K160	Ubiquitination	Uniprot
K162	Acetylation	Uniprot
K162	Ubiquitination	Uniprot
C174	S-Nitrosylation	Uniprot
T175	Phosphorylation	Uniprot
K178	Ubiquitination	Uniprot
K187	Ubiquitination	Uniprot
K188	Ubiquitination	Uniprot
S214	Phosphorylation	Uniprot
S217	Phosphorylation	Uniprot
K219	Ubiquitination	Uniprot
K223	Ubiquitination	Uniprot
S241	Phosphorylation	Uniprot
K242	Ubiquitination	Uniprot
K303	Ubiquitination	Uniprot
K314	Ubiquitination	Uniprot
K323	Ubiquitination	Uniprot
K324	Ubiquitination	Uniprot
K332	Ubiquitination	Uniprot
S335	Phosphorylation	Uniprot
K352	Ubiquitination	Uniprot
S394	Phosphorylation	Uniprot
K409	Ubiquitination	Uniprot
Y432	Phosphorylation	Uniprot
K441	Ubiquitination	Uniprot
S444	Phosphorylation	Uniprot
S455	Phosphorylation	Uniprot
R459	Methylation	Uniprot
Y466	Phosphorylation	Uniprot
K468	Ubiquitination	Uniprot

Research Backgrounds

P11172_UMPS_HUMAN

Subunit Structure:

Homodimer.

Family&Domains:

In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

In the C-terminal section; belongs to the OMP decarboxylase family.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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For Research Use Only.
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UMPS Antibody - #DF12790