TRPC1 Antibody - #DF12783

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Product: TRPC1 Antibody
Catalog: DF12783
Description: Rabbit polyclonal antibody to TRPC1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 91 kDa; 91kD(Calculated).
Uniprot: P48995
RRID: AB_2845744

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TRPC1 Antibody detects endogenous levels of total TRPC1.
RRID:
AB_2845744
Cite Format: Affinity Biosciences Cat# DF12783, RRID:AB_2845744.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HTRP 1; HTRP1; MGC133334; MGC133335; Mtrp1; Short transient receptor potential channel 1; Transient receptor potential canonical 1; Transient receptor potential cation channel subfamily C member 1; Transient receptor potential channel 1; Transient receptor protein 1; TRP 1; TRP 1 protein; Trp related protein 1; TRP-1; TRP1; TRP1 protein; TrpC1; Trpc1 transient receptor potential cation channel subfamily C member 1; TRPC1_HUMAN; Trrp1;

Immunogens

Immunogen:
Uniprot:

P48995(TRPC1_HUMAN) >>Visit HPA database.

Gene(ID):
TRPC1(7220)
Expression:
P48995 TRPC1_HUMAN:

Seems to be ubiquitous.

Sequence:
MMAALYPSTDLSGASSSSLPSSPSSSSPNEVMALKDVREVKEENTLNEKLFLLACDKGDYYMVKKILEENSSGDLNINCVDVLGRNAVTITIENENLDILQLLLDYGCQSADALLVAIDSEVVGAVDILLNHRPKRSSRPTIVKLMERIQNPEYSTTMDVAPVILAAHRNNYEILTMLLKQDVSLPKPHAVGCECTLCSAKNKKDSLRHSRFRLDIYRCLASPALIMLTEEDPILRAFELSADLKELSLVEVEFRNDYEELARQCKMFAKDLLAQARNSRELEVILNHTSSDEPLDKRGLLEERMNLSRLKLAIKYNQKEFVSQSNCQQFLNTVWFGQMSGYRRKPTCKKIMTVLTVGIFWPVLSLCYLIAPKSQFGRIIHTPFMKFIIHGASYFTFLLLLNLYSLVYNEDKKNTMGPALERIDYLLILWIIGMIWSDIKRLWYEGLEDFLEESRNQLSFVMNSLYLATFALKVVAHNKFHDFADRKDWDAFHPTLVAEGLFAFANVLSYLRLFFMYTTSSILGPLQISMGQMLQDFGKFLGMFLLVLFSFTIGLTQLYDKGYTSKEQKDCVGIFCEQQSNDTFHSFIGTCFALFWYIFSLAHVAIFVTRFSYGEELQSFVGAVIVGTYNVVVVIVLTKLLVAMLHKSFQLIANHEDKEWKFARAKLWLSYFDDKCTLPPPFNIIPSPKTICYMISSLSKWICSHTSKGKVKRQNSLKEWRNLKQKRDENYQKVMCCLVHRYLTSMRQKMQSTDQATVENLNELRQDLSKFRNEIRDLLGFRTSKYAMFYPRN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
83
Bovine
75
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48995 As Substrate

Site PTM Type Enzyme
S206 Phosphorylation
T229 Phosphorylation
S279 Phosphorylation
T289 Phosphorylation
S308 Phosphorylation
T347 Phosphorylation
Y368 Phosphorylation
T415 Phosphorylation
Y425 Phosphorylation
S752 Phosphorylation
T757 Phosphorylation

Research Backgrounds

Function:

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.

PTMs:

Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Seems to be ubiquitous.

Subunit Structure:

Interacts with TRPC4AP (By similarity). Homotetramer and heterotetramer with TRPC4 and/or TRPC5. Interacts with TRPC3, TRPC4 and TRPC5. Interacts with ITPR3. Interacts with MX1 and RNF24. Interacts with FKBP4.

Family&Domains:

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Targeting transient receptor potential canonical 1 reduces non‑small cell lung cancer chemoresistance and stemness via inhibition of PI3K/AKT signaling. Oncology Letters, 2023 (PubMed: 37153044) [IF=2.9]

Application: WB    Species: Human    Sample: NSCLC cells

Figure 1. TRPC1 expression in normal and CDDP-resistant NSCLC cells. The dose-response curve of CDDP in (A) A549 cells, (B) A549/CDDP cells, (C) H460 cells, and (D) H460/CDDP cells. (E) TRPC1 mRNA expression. (F) Representative western blots of TRPC1 expression. (G) Densitometry analysis of TRPC1 protein expression. *P
2). HDAC1 regulates inflammation and osteogenic differentiation of ankylosing spondylitis fibroblasts through the Wnt-Smad signaling pathway. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35794630) [IF=2.6]

Application: WB    Species: Human    Sample: AS fibroblasts

Fig. 4 Expression of TRPC1 and TRPV4 in AS fibroblasts after different treatment. A Protein expressions of TRPC1 and TRPV4 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of protein expression. C Representative images showing immunofluorescence staining of TRPC1. Magnification, × 400. D Representative images showing immunofluorescence staining of TRPV4. Magnification, × 400. E Mean fluorescence intensity of TRPC1. F Mean fluorescence intensity of TRPV4. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group

Application: IF/ICC    Species: Human    Sample: AS fibroblasts

Fig. 4 Expression of TRPC1 and TRPV4 in AS fibroblasts after different treatment. A Protein expressions of TRPC1 and TRPV4 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of protein expression. C Representative images showing immunofluorescence staining of TRPC1. Magnification, × 400. D Representative images showing immunofluorescence staining of TRPV4. Magnification, × 400. E Mean fluorescence intensity of TRPC1. F Mean fluorescence intensity of TRPV4. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group

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