Phospho-ACC1 (Ser79)[Ser80] Antibody

Product:	Phospho-ACC1 (Ser79)[Ser80] Antibody
Catalog:	AF3421
Description:	Rabbit polyclonal antibody to Phospho-ACC1 (Ser79)[Ser80]
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	280kDa; 266kD(Calculated).
Uniprot:	Q13085
RRID:	AB_2834863

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(85%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

Phospho-ACC1 (Ser79) Antibody detects endogenous levels of ACC1 only when phosphorylated at Ser80, which site historically referenced as Ser79.

RRID:

AB_2834863
Cite Format: Affinity Biosciences Cat# AF3421, RRID:AB_2834863.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAC;ACACA;ACACA_HUMAN;ACC alpha;ACC 1;ACC-alpha;ACC1;ACCA;Acetyl CoA carboxylase 1;Acetyl CoA carboxylase alpha;Acetyl Coenzyme A carboxylase alpha;Biotin carboxylase;COA1;HACC275 antibody;

Immunogens

Immunogen:

Uniprot:

Q13085(ACACA_HUMAN) >>Visit HPA database.

Gene(ID):

ACACA(31)

Expression:

Q13085 ACACA_HUMAN:

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

Description:

ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis.

Sequence:

Q13085 ACACA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Rabbit

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13085 As Substrate

Q13085

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
S23	Phosphorylation		Uniprot
S25	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
S34	Phosphorylation		Uniprot
K45	Ubiquitination		Uniprot
S48	Phosphorylation		Uniprot
S50	Phosphorylation		Uniprot
S53	Phosphorylation		Uniprot
S56	Phosphorylation		Uniprot
T58	Phosphorylation		Uniprot
S60	Phosphorylation		Uniprot
S77	Phosphorylation	P17612 (PRKACA)	Uniprot
S78	Phosphorylation	P54646 (PRKAA2)	Uniprot
S80	Phosphorylation	P54646 (PRKAA2) , Q13131 (PRKAA1)	Uniprot
K86	Ubiquitination		Uniprot
S96	Phosphorylation		Uniprot
S104	Phosphorylation		Uniprot
K116	Ubiquitination		Uniprot
K120	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
S234	Phosphorylation		Uniprot
K276	Ubiquitination		Uniprot
K288	Ubiquitination		Uniprot
Y290	Phosphorylation		Uniprot
K292	Ubiquitination		Uniprot
K311	Ubiquitination		Uniprot
S344	Phosphorylation		Uniprot
K386	Ubiquitination		Uniprot
K410	Ubiquitination		Uniprot
Y417	Phosphorylation		Uniprot
Y434	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
K520	Ubiquitination		Uniprot
K579	Ubiquitination		Uniprot
T610	Phosphorylation		Uniprot
Y676	Phosphorylation		Uniprot
Y734	Phosphorylation		Uniprot
K747	Ubiquitination		Uniprot
S756	Phosphorylation		Uniprot
K817	Ubiquitination		Uniprot
K825	Ubiquitination		Uniprot
S835	Phosphorylation		Uniprot
S841	Phosphorylation		Uniprot
Y855	Phosphorylation		Uniprot
Y867	Phosphorylation		Uniprot
K879	Ubiquitination		Uniprot
S893	Phosphorylation		Uniprot
S907	Phosphorylation		Uniprot
K916	Ubiquitination		Uniprot
S977	Phosphorylation		Uniprot
Y994	Phosphorylation		Uniprot
T1042	Phosphorylation		Uniprot
T1073	Phosphorylation		Uniprot
K1076	Ubiquitination		Uniprot
T1196	Phosphorylation		Uniprot
S1201	Phosphorylation	P17612 (PRKACA)	Uniprot
Y1209	Phosphorylation		Uniprot
S1216	Phosphorylation		Uniprot
S1218	Phosphorylation		Uniprot
T1227	Phosphorylation		Uniprot
S1257	Phosphorylation		Uniprot
S1259	Phosphorylation		Uniprot
S1263	Phosphorylation		Uniprot
T1265	Phosphorylation		Uniprot
S1273	Phosphorylation		Uniprot
K1316	Ubiquitination		Uniprot
K1334	Acetylation		Uniprot
K1334	Ubiquitination		Uniprot
K1338	Ubiquitination		Uniprot
Y1342	Phosphorylation		Uniprot
K1354	Ubiquitination		Uniprot
Y1370	Phosphorylation		Uniprot
K1435	Ubiquitination		Uniprot
S1438	Phosphorylation		Uniprot
Y1441	Phosphorylation		Uniprot
Y1499	Phosphorylation		Uniprot
K1524	Ubiquitination		Uniprot
K1580	Acetylation		Uniprot
K1586	Ubiquitination		Uniprot
S1720	Phosphorylation		Uniprot
Y1745	Phosphorylation		Uniprot
K1759	Acetylation		Uniprot
K1759	Ubiquitination		Uniprot
K1781	Ubiquitination		Uniprot
K1788	Acetylation		Uniprot
K1788	Ubiquitination		Uniprot
S1844	Phosphorylation		Uniprot
K1856	Acetylation		Uniprot
K1856	Ubiquitination		Uniprot
S1908	Phosphorylation		Uniprot
K1916	Ubiquitination		Uniprot
K1929	Ubiquitination		Uniprot
K2031	Acetylation		Uniprot
K2031	Ubiquitination		Uniprot
R2047	Methylation		Uniprot
S2099	Phosphorylation		Uniprot
Y2108	Phosphorylation		Uniprot
K2127	Acetylation		Uniprot
K2127	Ubiquitination		Uniprot
K2135	Ubiquitination		Uniprot
Y2144	Phosphorylation		Uniprot
T2153	Phosphorylation		Uniprot
K2209	Ubiquitination		Uniprot
K2231	Ubiquitination		Uniprot
K2268	Ubiquitination		Uniprot
K2293	Ubiquitination		Uniprot
K2302	Ubiquitination		Uniprot
S2318	Phosphorylation		Uniprot
S2343	Phosphorylation		Uniprot
S2345	Phosphorylation		Uniprot

Research Backgrounds

Q13085_ACACA_HUMAN

Function:

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.

PTMs:

Phosphorylation on Ser-1263 is required for interaction with BRCA1.

Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.

The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.

Subcellular Location:

Cytoplasm>Cytosol.

Tissue Specificity:

Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

Subunit Structure:

Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.

Family&Domains:

Consists of an N-terminal biotin carboxylation/carboxylase (BC) domain that catalyzes the ATP-dependent transient carboxylation of the biotin covalently attached to the central biotinyl-binding/biotin carboxyl carrier (BCC) domain (Probable). The C-terminal carboxyl transferase (CT) domain catalyzes the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA to produce malonyl-CoA (Probable).

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Comprehensive multi-omics approaches reveal the hepatotoxic mechanism of perfluorohexanoic acid (PFHxA) in mice. Science of The Total Environment, 2021 (PubMed: 34380288) [IF=9.8]

Application: IHC Species: Mice Sample: liver tissue

Fig. 4. Expression analysis of genes and proteins associated with fatty acid metabolism in mice with PFHxA exposure. (A) Expression of fatty acid biosynthesis genes, (B) Expression of fatty acid peroxisomal oxidation genes, (C) Expression of genes related to inflammation. (D–G) IHC staining of FASN (D), p-ACAC (E), EHHADH (F) and p-mTOR (G). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group (n = 3–4).

2). Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells. Biomedicine & Pharmacotherapy, 2020 (PubMed: 33152903) [IF=7.5]

3). Ginsenoside CK ameliorates hepatic lipid accumulation via activating the LKB1/AMPK pathway in vitro and in vivo. Food & Function, 2022 (PubMed: 35018944) [IF=6.1]

4). α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube. Nutrients, 2021 (PubMed: 34371902) [IF=5.9]

Application: WB Species: Mice Sample:

Figure 2 The effects of HICA on the intracellular signaling pathways. A typical image for a capillary immunoassay is shown (A). The phosphorylation levels of (B) p70S6K and 4E-BP1; (C) AMPK, ACC, and ULK1; (D) ERK1/2; (E) p38MAPK; and (F) eEF2 are shown. The phosphorylation is normalized to the total protein expression. The β-tubulin content in the lysate was measured as a loading control (G). The time course of these experiments is shown in the upper region. DM: differentiation medium, DMEM: Dulbecco’s modified Eagle’s medium, and w/o AA: without amino acids. Data are displayed as the means ± SD, and n = 4 for each group in all bar graphs. * p < 0.05 and ** p < 0.01 vs. the vehicle-treated group.

5). Amphibian pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell nutrient deficiency. iScience, 2022 (PubMed: 37128610) [IF=5.8]

6). Liraglutide Alleviates Hepatic Steatosis and Liver Injury in T2MD Rats via a GLP-1R Dependent AMPK Pathway. Frontiers in Pharmacology, 2021 (PubMed: 33746742) [IF=5.6]

7). Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells. Journal of Functional Foods, 2021 [IF=5.6]

8). Cai's herbal tea enhances mitochondrial autophagy of type 1 diabetic mellitus β cells through the AMPK/mTOR pathway and alleviates inflammatory response. Acta diabetologica, 2024 (PubMed: 38954041) [IF=3.8]

9). Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid. The British journal of nutrition, 2022 (PubMed: 33910655) [IF=3.6]

Application: WB Species: Crab Sample: hepatopancreas tissues

Fig. 5. Effects of dietary myo-inositol on protein levels of p-IP3R, p-AMPK and p-ACC1 of Eriocheir sinensis fed different lipid level. Values are means (n=4 replicate tanks) with standard errors represented by vertical bars. * indicates significant difference between myo-inositol levels within the same lipid level or between lipid levels within the same myo-inositol level (P < 0.05). p-IP3R, phosphorylation of inositol 1,4,5-trisphosphate receptor; p-AMPK, phosphorylation of adenosine 5’-monophosphate-activated protein kinase; p-ACC1, phosphorylation of acetyl-CoA carboxylase 1.

10). Liraglutide regulates lipid metabolism via FGF21-LKB1-AMPK-ACC1 pathway in white adipose tissues and macrophage of type 2 diabetic mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 33640604) [IF=3.1]

Application: WB Species: mice Sample: White adipose tissue (WAT)

Fig. 2. Effects of LRG on adipocyte size and the protein expression in WAT of diabetic mice. (A) Adipocyte size of the mice; (B) Quantification of Fig. 2A; (C) Immunohistochemical assay of WAT; (D) Quantification of Fig. 2C; (E) Protein expression in WAT; (F) Quantification of Fig. 3E n ¼ 6 mice/group. #p < 0.05, ##p < 0.01,###p < 0.001 vs CON; *p < 0.05,**p < 0.01, ***p < 0.001 vs DM.

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Phospho-ACC1 (Ser79)[Ser80] Antibody - #AF3421