Phospho-ACC1 (Ser79)[Ser80] Antibody - #AF3421
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![Phospho-ACC1 (Ser79)[Ser80] Antibody - Western blot analysis of extracts from RAW264.](http://img.affbiotech.cn/images/202106/thumb_img/af3421_phospho_acc1_ser79_ser80_antibody_thumb_P55460_1623820767831.jpg "Western blot analysis of extracts from RAW264.7 cells, using Phospho-ACC1 (Ser79)[Ser80] Antibody. The lane on the left was treated with blocking peptide.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Mouse skin tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457438.jpg "AF3421 at 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat colon tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457595.jpg "AF3421 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat stomach tissue by IHC-P.](http://img.affbiotech.cn/images/202005/thumb_img/af3421_phospho_acc1_ser79_ser80_antibody_thumb_P40978_1588836607009.jpg "AF3421 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat ovary tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457583.jpg "AF3421 at 1/100 staining Rat ovary tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat kidney tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457270.jpg "AF3421 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Human lung cancer by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457710.jpg "AF3421 at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Human mammary cancer by IHC-P.](http://img.affbiotech.cn/images/202005/thumb_img/af3421_phospho_acc1_ser79_ser80_antibody_thumb_P40974_1588836607780.jpg "AF3421 at 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Human mammary cancer by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457043.jpg "AF3421 at 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Human colorectal cancer by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457117.jpg "AF3421 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Human colorectal cancer by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999457566.jpg "AF3421 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/200 staining human liver cancer tissue sections by IHC-P.](http://img.affbiotech.cn/images/201711/thumb_img/1609_thumb_P_1510711468478.jpg "AF3421 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Mouse muscle tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456015.jpg "AF3421 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Mouse testis tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456276.jpg "AF3421 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat liver tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456883.jpg "AF3421 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Mouse kidney tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456985.jpg "AF3421 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Mouse spleen tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456771.jpg "AF3421 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Mouse heart tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456047.jpg "AF3421 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat skin tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456743.jpg "AF3421 at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat brain tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456239.jpg "AF3421 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat thymus tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456951.jpg "AF3421 at 1/100 staining Rat thymus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 at 1/100 staining Rat heart tissue by IHC-P.](http://img.affbiotech.cn/images/201911/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1573999456941.jpg "AF3421 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - AF3421 staining H2O2 treated A549 by IF/ICC.](http://img.affbiotech.cn/images/201912/thumb_img/af3421_phospho_acc_ser80_antibody_thumb_P_1577151531736.jpg "AF3421 staining H2O2 treated A549 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF3421) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI (blue).")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - peptide-ELISA analysis of AF3421.](http://img.affbiotech.cn/images/pelisa/809/af3421-peptide-elisa.png "peptide-ELISA analysis of AF3421. showing specificity to antigen peptide. Peptides concentration: 1ug/ml.<br>
P-peptide: phospho-peptide. N-peptide: non-phospho-peptide.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - Fig.](http://img.affbiotech.cn/images/cited_image/202201/cite-125-1641550585.jpg "Fig. 2. Effects of LRG on adipocyte size and the protein expression in WAT of diabetic mice. (A) Adipocyte size of the mice; (B) Quantification of Fig. 2A; (C) Immunohistochemical assay of WAT; (D) Quantification of Fig. 2C; (E) Protein expression in WAT; (F) Quantification of Fig. 3E n ¼ 6 mice/group. #p < 0.05, ##p < 0.01,###p < 0.001 vs CON;
*p < 0.05,**p < 0.01, ***p < 0.001 vs DM.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - Fig.](http://img.affbiotech.cn/uploads/202309/dfe87897db3d7d027fae3e3556491a14.png "Fig. 1 AMPK mediated the synovioprotective effect of L-carnitine in an LPS-stimulated FLS model. a HE and anti-vimentin immunostaining of FLS for cell identification. b The cell viability of L-carnitine on FLS. c Toxicity was detected by LDH. d ELISA measurement of IL-1β, IL-6, IL-8, TNF-α, and IL-10 levels in the supernatant. e Immunostaining shows that pAMPK is increased after L-carnitine administration (× 630 magnification). f ATP detection shows that L-carnitine can replenish ATP levels. g Immunoblot analysis of AMPK, pAMPK, ACC, and pACC. h Real-time PCR data of AMPK and ACC. Data are presented as the mean ± SD (n = 3). ns, not significant.")
![Phospho-ACC1 (Ser79)[Ser80] Antibody - Fig.](http://img.affbiotech.cn/uploads/202401/9cb8340c7a1bceb63a75bf35e26b907d.png "Fig. S2. Compound C and SBI-0206965 inhibit activation of AMPK signaling in toad liver cells under Glc-/Gln- conditions. (A) Sequence alignment of AMPKs (Top) and ACCs (Bottom) in toad B. maxima and other species was analyzed by Clustal Omega6. The activation loop of AMPK is indicated by a green line. The phosphorylated site (Thr residue of AMPKs and Ser residue of ACCs) is indicated by a red spot. (B) AMPK signaling activation in toad liver cells after culture in Glc+/Gln+, Glc-/Gln-, or Glc+/Gln- medium for 3 hours was detected by western blotting. (C, D) Cytotoxicity of compound C (C) and SBI-0206965 (D) in toad liver cells was detected by PI staining after treatment with compound C or SBI-0206965 in Glc-/Gln- medium for 3 hours. (E) Phosphorylation of pS79-ACC1 in toad liver cells was determined by western blotting after treatment with compound C or SBI-0206965 in Glc-/Gln- medium for 3 hours. Data (C, D) are represented as the mean ± SD of triplicate samples. ns (P≥0.05) by one-way ANOVA. All data are representative of at least two independent experiments.")
| Product: | Phospho-ACC1 (Ser79)[Ser80] Antibody |
| Catalog: | AF3421 |
| Description: | Rabbit polyclonal antibody to Phospho-ACC1 (Ser79)[Ser80] |
| Application: | WB IHC IF/ICC |
| Cited expt.: | WB, IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 280kDa; 266kD(Calculated). |
| Uniprot: | Q13085 |
| RRID: | AB_2834863 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3421, RRID:AB_2834863.
Fold/Unfold
ACAC;ACACA;ACACA_HUMAN;ACC alpha;ACC 1;ACC-alpha;ACC1;ACCA;Acetyl CoA carboxylase 1;Acetyl CoA carboxylase alpha;Acetyl Coenzyme A carboxylase alpha;Biotin carboxylase;COA1;HACC275 antibody;
Immunogens
A synthesized peptide derived from human ACC1 around the phosphorylation site of Ser79.
Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
- Q13085 ACACA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFLVAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Cytoplasm>Cytosol.
Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Consists of an N-terminal biotin carboxylation/carboxylase (BC) domain that catalyzes the ATP-dependent transient carboxylation of the biotin covalently attached to the central biotinyl-binding/biotin carboxyl carrier (BCC) domain (Probable). The C-terminal carboxyl transferase (CT) domain catalyzes the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA to produce malonyl-CoA (Probable).
Research Fields
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Fatty acid biosynthesis.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Fatty acid metabolism.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
References
Application: IHC Species: Mice Sample: liver tissue
Application: WB Species: Mice Sample:
Application: WB Species: Crab Sample: hepatopancreas tissues
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