TH1L Antibody - #DF12771
| Product: | TH1L Antibody |
| Catalog: | DF12771 |
| Description: | Rabbit polyclonal antibody to TH1L |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 66 kDa; 66kD(Calculated). |
| Uniprot: | Q8IXH7 |
| RRID: | AB_2845732 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12771, RRID:AB_2845732.
Fold/Unfold
HSPC130; Negative elongation factor C/D; NELF C; NELF D; NELF-C/D; NELFC; NELFD; NELFD_HUMAN; TH1; TH1-like protein; TH1L;
Immunogens
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas.
- Q8IXH7 NELFD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAVPGAIMDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHILAYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIAGTIKTEGEHDPVTEFIAHCKSNFIMVN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IXH7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S34 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Uniprot | |
| Y56 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| Y289 | Phosphorylation | Uniprot | |
| R291 | Methylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K372 | Acetylation | Uniprot | |
| K374 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| K567 | Sumoylation | Uniprot |
Research Backgrounds
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
Nucleus.
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas.
The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; NELFA and NELFCD form a stable subcomplex that binds primarily through NELFCD to the N-terminus of NELFB. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner. Interacts with ARAF1. Interacts with PCF11. Interacts with KAT8 (By similarity).
Belongs to the NELF-D family.
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