Syntaxin4 Antibody - #DF12761

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Product: Syntaxin4 Antibody
Catalog: DF12761
Description: Rabbit polyclonal antibody to Syntaxin4
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 35 kDa, 40 kDa; 34kD(Calculated).
Uniprot: Q12846
RRID: AB_2845722

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Syntaxin4 Antibody detects endogenous levels of total Syntaxin4.
RRID:
AB_2845722
Cite Format: Affinity Biosciences Cat# DF12761, RRID:AB_2845722.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NY REN 31 antigen; p35 2; PLACENTAL; Renal carcinoma antigen NY REN 31; Renal carcinoma antigen NY-REN-31; Stx4; STX4_HUMAN; STX4A; Syntaxin 4; syntaxin 4A (placental); Syntaxin 4A; Syntaxin-4;

Immunogens

Immunogen:
Uniprot:

Q12846(STX4_HUMAN) >>Visit HPA database.

Gene(ID):
STX4(6810)
Expression:
Q12846 STX4_HUMAN:

Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.

Sequence:
MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVLIAICVSITVVLLAVIIGVTVVG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
92
Zebrafish
50
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12846 As Substrate

Site PTM Type Enzyme
Phosphorylation
S14 Phosphorylation
S15 Phosphorylation
T31 Phosphorylation
S36 Phosphorylation
K44 Ubiquitination
T51 Phosphorylation
K54 Ubiquitination
K58 Ubiquitination
K64 Ubiquitination
T72 Phosphorylation
S78 Phosphorylation
K80 Ubiquitination
K91 Ubiquitination
K102 Ubiquitination
K108 Ubiquitination
Y115 Phosphorylation
S117 Phosphorylation
T120 Phosphorylation
K124 Ubiquitination
T125 Phosphorylation
K140 Ubiquitination
S169 Phosphorylation
S204 Phosphorylation
S208 Phosphorylation
K243 Ubiquitination
S248 Phosphorylation
Y251 Phosphorylation
K260 Ubiquitination

Research Backgrounds

Function:

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.

Subcellular Location:

Cell membrane>Single-pass type IV membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.

Subunit Structure:

Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that interacts with SYT7 during lysosomal exocytosis. Found in a complex with VAMP8 and SNAP23. Detected in a complex with SNAP23 and STXBP4. Interacts with VAMP2. Interacts with SNAP23 and SNAPIN. Interacts with LLGL1. Interacts (via C-terminus) with CENPF. Interacts with DOC2B. Interacts with STXBP6. Interacts with STXBP3; excludes interaction with DOC2B and SNAP25. Interacts with STXBP4; excludes interaction with VAMP2 (By similarity). Interacts with STXBP5L (By similarity).

Family&Domains:

Belongs to the syntaxin family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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