Product: SPTLC1 Antibody
Catalog: DF12752
Description: Rabbit polyclonal antibody to SPTLC1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 55 kDa; 53kD(Calculated).
Uniprot: O15269
RRID: AB_2845713

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SPTLC1 Antibody detects endogenous levels of total SPTLC1.
RRID:
AB_2845713
Cite Format: Affinity Biosciences Cat# DF12752, RRID:AB_2845713.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HSAN; HSAN1; HSN1; LBC1; LCB 1; LCB1; Long chain base biosynthesis protein 1; MGC14645; Serine C palmitoyltransferase; Serine palmitoyl CoA transferase 1; Serine palmitoyltransferase 1; Serine palmitoyltransferase long chain base subunit 1; Serine palmitoyltransferase subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTC1_HUMAN; SPTI; SPTLC 1; SPTLC1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15269 SPTC1_HUMAN:

Widely expressed. Not detected in small intestine.

Sequence:
MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYGTFDVHLDLEDRLAKFMKTEEAIIYSYGFATIASAIPAYSKRGDIVFVDRAACFAIQKGLQASRSDIKLFKHNDMADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPGIFAVLKEKCGQIHKALQGISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCMNRSIALTQARYLEKEEKCLPPPSIRVVVTVEQTEEELERAASTIKEVAQAVLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15269 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
S50 Phosphorylation
T53 Phosphorylation
K55 Ubiquitination
K57 Ubiquitination
K75 Ubiquitination
Y82 Phosphorylation
K92 Ubiquitination
K118 Ubiquitination
K126 Ubiquitination
K127 Ubiquitination
Y164 Phosphorylation P00519 (ABL1)
K197 Ubiquitination
K207 Ubiquitination
K210 Methylation
K210 Ubiquitination
K222 Ubiquitination
K230 Ubiquitination
K369 Ubiquitination
K375 Ubiquitination
K384 Ubiquitination
S389 Phosphorylation
S391 Phosphorylation
K434 Acetylation
K434 Ubiquitination
C438 S-Nitrosylation
S443 Phosphorylation
T453 Phosphorylation
K465 Ubiquitination

Research Backgrounds

Function:

Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis (By similarity).

PTMs:

Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Not detected in small intestine.

Subunit Structure:

Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. The composition of the complex will define the substrate specificity. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3. Interacts with RTN4 (isoform B) (By similarity).

Family&Domains:

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Notoginsenoside R1 Ameliorates Cardiac Lipotoxicity Through AMPK Signaling Pathway. Frontiers in Pharmacology, 2022 (PubMed: 35370720) [IF=5.6]

Application: WB    Species: Mice    Sample: H9C2 cells

FIGURE 3 NGR1 regulated lipotoxicity-related proteins and reduced apoptosis in the HF mice model. (A-C)Western blot analysis of p-AMPK, AMPK, GPAT, SPTLC1, SPTLC2, CPT-1A, Bcl-2 and Bax in each group of mice. GAPDH served as the internal control, *p < 0.05, **p < 0.01 vs. model group, # p < 0.05, ## p < 0.01, ### p < 0.001 vs. sham group, ns stands for not statistically significant, N = 3-5 per group.

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