SPTLC1 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from Jurkat cells, using SPTLC1 Antibody.

Western blot analysis of extracts from HepG2 cells, using SPTLC1 Antibody.

DF12752 staining Hela cells by IF/ICC.

FIGURE 3 NGR1 regulated lipotoxicity-related proteins and reduced apoptosis in the HF mice model.

Product:	SPTLC1 Antibody
Catalog:	DF12752
Description:	Rabbit polyclonal antibody to SPTLC1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	55 kDa; 53kD(Calculated).
Uniprot:	O15269
RRID:	AB_2845713

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SPTLC1 Antibody detects endogenous levels of total SPTLC1.

RRID:

AB_2845713
Cite Format: Affinity Biosciences Cat# DF12752, RRID:AB_2845713.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HSAN; HSAN1; HSN1; LBC1; LCB 1; LCB1; Long chain base biosynthesis protein 1; MGC14645; Serine C palmitoyltransferase; Serine palmitoyl CoA transferase 1; Serine palmitoyltransferase 1; Serine palmitoyltransferase long chain base subunit 1; Serine palmitoyltransferase subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTC1_HUMAN; SPTI; SPTLC 1; SPTLC1;

Immunogens

Immunogen:

Uniprot:

O15269(SPTC1_HUMAN) >>Visit HPA database.

Gene(ID):

SPTLC1(10558)

Expression:

O15269 SPTC1_HUMAN:

Widely expressed. Not detected in small intestine.

Sequence:

O15269 SPTC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYGTFDVHLDLEDRLAKFMKTEEAIIYSYGFATIASAIPAYSKRGDIVFVDRAACFAIQKGLQASRSDIKLFKHNDMADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPGIFAVLKEKCGQIHKALQGISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCMNRSIALTQARYLEKEEKCLPPPSIRVVVTVEQTEEELERAASTIKEVAQAVLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15269 As Substrate

O15269

Site	PTM Type	Enzyme	Source
K45	Ubiquitination		Uniprot
S50	Phosphorylation		Uniprot
T53	Phosphorylation		Uniprot
K55	Ubiquitination		Uniprot
K57	Ubiquitination		Uniprot
K75	Ubiquitination		Uniprot
Y82	Phosphorylation		Uniprot
K92	Ubiquitination		Uniprot
K118	Ubiquitination		Uniprot
K126	Ubiquitination		Uniprot
K127	Ubiquitination		Uniprot
Y164	Phosphorylation	P00519 (ABL1)	Uniprot
K197	Ubiquitination		Uniprot
K207	Ubiquitination		Uniprot
K210	Methylation		Uniprot
K210	Ubiquitination		Uniprot
K222	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
K369	Ubiquitination		Uniprot
K375	Ubiquitination		Uniprot
K384	Ubiquitination		Uniprot
S389	Phosphorylation		Uniprot
S391	Phosphorylation		Uniprot
K434	Acetylation		Uniprot
K434	Ubiquitination		Uniprot
C438	S-Nitrosylation		Uniprot
S443	Phosphorylation		Uniprot
T453	Phosphorylation		Uniprot
K465	Ubiquitination		Uniprot

Research Backgrounds

O15269_SPTC1_HUMAN

Function:

Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis (By similarity).

PTMs:

Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Tissue Specificity:

Widely expressed. Not detected in small intestine.

Subunit Structure:

Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. The composition of the complex will define the substrate specificity. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3. Interacts with RTN4 (isoform B) (By similarity).

Family&Domains:

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Notoginsenoside R1 Ameliorates Cardiac Lipotoxicity Through AMPK Signaling Pathway. Frontiers in Pharmacology, 2022 (PubMed: 35370720) [IF=5.6]

Application: WB Species: Mice Sample: H9C2 cells

FIGURE 3 NGR1 regulated lipotoxicity-related proteins and reduced apoptosis in the HF mice model. (A-C)Western blot analysis of p-AMPK, AMPK, GPAT, SPTLC1, SPTLC2, CPT-1A, Bcl-2 and Bax in each group of mice. GAPDH served as the internal control, *p < 0.05, **p < 0.01 vs. model group, # p < 0.05, ## p < 0.01, ### p < 0.001 vs. sham group, ns stands for not statistically significant, N = 3-5 per group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SPTLC1 Antibody - #DF12752