SPRR3 Antibody - #DF12751
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(4)
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
SPRR3 Antibody detects endogenous levels of total SPRR3.
RRID:
AB_2845712
Cite Format: Affinity Biosciences Cat# DF12751, RRID:AB_2845712.
Cite Format: Affinity Biosciences Cat# DF12751, RRID:AB_2845712.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
22 kDa pancornulin; Cornifin beta; Esophagin; Small proline rich protein 3; Small proline-rich protein 3; SPRC; Sprr3; SPRR3_HUMAN;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9UBC9 SPRR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKLPEPCPSTVTPGPAQQKTKQK
PTMs - Q9UBC9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| Y106 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Cross-linked envelope protein of keratinocytes.
Subcellular Location:
Cytoplasm.
Family&Domains:
Belongs to the cornifin (SPRR) family.
References
1). SPRR3, a novel miR‑338‑3p target, regulates the malignant progression of clear cell renal cell carcinoma in vitro via the PI3K/Akt signaling pathway. Experimental and Therapeutic Medicine, 2022
(PubMed: 35350667)
[IF=2.7]
Application: WB Species: Human Sample: ccRCC cells
Figure 1
Association between SPRR3 expression and the prognosis of ccRCC, and detection of SPRR3 expression in human normal renal cells HK-2 and ccRCC cell lines. (A) Survival analysis of 531 patients with ccRCC by Kaplan-Meier curve (P=0.0017). SPRR3 expression in HK-2, 786-O, CaKi-1 and UMRC-2 cell lines, as detected using (B) reverse transcription-quantitative PCR and (C) western blotting. **P<0.01 vs. HK-2. SPRR3, small proline-rich repeat protein 3; ccRCC, clear cell renal cell carcinoma.
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