Product: SPCS1 Antibody
Catalog: DF12750
Description: Rabbit polyclonal antibody to SPCS1
Application: WB
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 12 kDa; 18kD(Calculated).
Uniprot: Q9Y6A9
RRID: AB_2845711

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
SPCS1 Antibody detects endogenous levels of total SPCS1.
RRID:
AB_2845711
Cite Format: Affinity Biosciences Cat# DF12750, RRID:AB_2845711.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HSPC033; Microsomal signal peptidase 12 kDa subunit; OTTHUMP00000206959; signal peptidase 12kDa; Signal peptidase complex subunit 1; signal peptidase complex subunit 1 homolog (S. cerevisiae); SPase 12 kDa subunit; SPC1; SPC12; SPCS1; SPCS1_HUMAN; YJR010C-A;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MARGGDTGCTGPSETSASGAAAIALPGLEGPATDAQCQTLPLTVLKSRSPSPRSLPPALSCPPPQPAMLEHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQESSTDDKKPGERKIKRHAKNN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Zebrafish
83
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6A9 As Substrate

Site PTM Type Enzyme
Y14 Phosphorylation
K15 Ubiquitination
K18 Ubiquitination
K76 Ubiquitination
S83 Phosphorylation
S84 Phosphorylation
K89 Ubiquitination
K94 Acetylation
K100 Acetylation

Research Backgrounds

Function:

Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.

(Microbial infection) Required for the post-translational processing of proteins involved in virion assembly and secretion from flaviviruses such as West Nile virus (WNV), Japanese encephalitis virus (JEV), Dengue virus type 2 (DENV-2), Yellow Fever virus (YFV), Zika virus (ZIKV) and hepatitis C virus (HCV). Plays a key role in the post-translational processing of flaviviral structural proteins prM, E, and NS1. In HCV, it is involved in virion assembly where it promotes the interaction between HCV virus proteins NS2 and E2.

Subcellular Location:

Microsome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3.

(Microbial infection) Interacts with hepatitis C virus (HCV) proteins NS2 and E2. Interacts with NS2B from Japanese encephalitis virus (JEV), West Nile virus (WNV), and Zika virus (ZIKV).

Family&Domains:

Belongs to the SPCS1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein export.

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For Research Use Only.
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