SNAP29 Antibody - #DF12743

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Product: SNAP29 Antibody
Catalog: DF12743
Description: Rabbit polyclonal antibody to SNAP29
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Rabbit
Mol.Wt.: 29 kDa; 29kD(Calculated).
Uniprot: O95721
RRID: AB_2845704

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Rabbit(92%)
Clonality:
Polyclonal
Specificity:
SNAP29 Antibody detects endogenous levels of total SNAP29.
RRID:
AB_2845704
Cite Format: Affinity Biosciences Cat# DF12743, RRID:AB_2845704.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CEDNIK; FLJ21051; SNAP 29; SNAP-29; SNAP29; SNP29_HUMAN; Soluble 29 kDa NSF attachment protein; Synaptosomal associated protein 29; Synaptosomal associated protein 29kDa; Synaptosomal-associated protein 29; Vesicle membrane fusion protein SNAP 29; Vesicle membrane fusion protein SNAP29; Vesicle-membrane fusion protein SNAP-29;

Immunogens

Immunogen:
Uniprot:

O95721(SNP29_HUMAN) >>Visit HPA database.

Gene(ID):
SNAP29(9342)
Expression:
O95721 SNP29_HUMAN:

Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

Sequence:
MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Bovine
83
Pig
67
Dog
67
Horse
56
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95721 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
S7 Phosphorylation
Y8 Phosphorylation
R29 Methylation
R41 Methylation
Y44 Phosphorylation
T56 Phosphorylation
S59 Phosphorylation
T60 Phosphorylation
S61 Phosphorylation
S63 Phosphorylation
Y68 Phosphorylation
S77 Phosphorylation
S78 Phosphorylation
K103 Acetylation
K103 Ubiquitination
S105 Phosphorylation
K107 Ubiquitination
S111 Phosphorylation
K113 Ubiquitination
S114 Phosphorylation
Y122 Phosphorylation
K126 Ubiquitination
T130 Phosphorylation
T137 Phosphorylation
K147 Ubiquitination
S153 Phosphorylation
K154 Acetylation
K154 Ubiquitination
K159 Ubiquitination
Y160 Phosphorylation
S163 Phosphorylation
K169 Ubiquitination
S182 Phosphorylation
S185 Phosphorylation
T186 Phosphorylation
Y189 Phosphorylation
K191 Ubiquitination
S204 Phosphorylation
S210 Phosphorylation
K240 Ubiquitination
K249 Ubiquitination

Research Backgrounds

Function:

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Plays also a role in ciliogenesis by regulating membrane fusions.

Subcellular Location:

Cytoplasm. Golgi apparatus membrane>Peripheral membrane protein. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein. Cell projection>Cilium membrane>Peripheral membrane protein.
Note: Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. Localizes to the ciliary pocket from where the cilium protrudes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

Subunit Structure:

Forms a SNARE complex, composed of VAMP8, SNAP29 and STX17, involved in fusion of autophagosome with lysosome. Interacts with multiple syntaxins including STX6 (By similarity). Interacts with EIPR1.

Family&Domains:

Belongs to the SNAP-25 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

References

1). mTOR deletion ameliorates CD4+ T cell apoptosis during sepsis by improving autophagosome-lysosome fusion. APOPTOSIS, 2022 (PubMed: 35435531) [IF=7.2]

Application: WB    Species: Mice    Sample: CD4 + T cells

Fig. 3 Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4 + T cells were quantified by western blotting in wild-type (WT) sham-treated mice, WT CLP mice, mammalian target of rapamycin (mTOR) knockout via Cre expression under the control of lymphocyte-specific protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice, and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice. The amount of each protein level was normalized to that of β-actin. Means ± standard deviations (SDs) of four mice per group are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Application: WB    Species: mouse    Sample: CD4+T cells

Fig. 3| Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4+T cells were quantifed by western blotting in wild-type (WT)sham-treated mice, WT CLP mice, mammalian target of rapamycin(mTOR) knockout via Cre expression under the control of lymphocyte-specifc protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice,and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice.
2). Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis. Nutrients, 2022 (PubMed: 35277017) [IF=5.9]

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