SLC11A2 Antibody - #DF12740

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Product: SLC11A2 Antibody
Catalog: DF12740
Description: Rabbit polyclonal antibody to SLC11A2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse
Mol.Wt.: 65 kDa; 62kD(Calculated).
Uniprot: P49281
RRID: AB_2845701

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(83%)
Clonality:
Polyclonal
Specificity:
SLC11A2 Antibody detects endogenous levels of total SLC11A2.
RRID:
AB_2845701
Cite Format: Affinity Biosciences Cat# DF12740, RRID:AB_2845701.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DCT 1; dct-1; DCT1; Divalent cation transporter 1; Divalent metal transporter 1; DMT 1; DMT-1; DMT1; FLJ37416; Natural resistance associated macrophage protein 2; Natural resistance-associated macrophage protein 2; NRAM2_HUMAN; NRAMP 2; NRAMP2; OK/SW-cl.20; Slc11a2; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2; Solute carrier family 11 member 2;

Immunogens

Immunogen:
Uniprot:

P49281(NRAM2_HUMAN) >>Visit HPA database.

Gene(ID):
SLC11A2(4891)
Expression:
P49281 NRAM2_HUMAN:

Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.

Sequence:
MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKLWAFTGPGFLMSIAYLDPGNIESDLQSGAVAGFKLLWILLLATLVGLLLQRLAARLGVVTGLHLAEVCHRQYPKVPRVILWLMVELAIIGSDMQEVIGSAIAINLLSVGRIPLWGGVLITIADTFVFLFLDKYGLRKLEAFFGFLITIMALTFGYEYVTVKPSQSQVLKGMFVPSCSGCRTPQIEQAVGIVGAVIMPHNMYLHSALVKSRQVNRNNKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFGKTNEQVVEVCTNTSSPHAGLFPKDNSTLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTYSGQFVMEGFLNLKWSRFARVVLTRSIAIIPTLLVAVFQDVEHLTGMNDFLNVLQSLQLPFALIPILTFTSLRPVMSDFANGLGWRIAGGILVLIICSINMYFVVVYVRDLGHVALYVVAAVVSVAYLGFVFYLGWQCLIALGMSFLDCGHTCHLGLTAQPELYLLNTMDADSLVSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Bovine
83
Rabbit
75
Pig
67
Dog
67
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49281 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation
S23 Phosphorylation
Y31 Phosphorylation
S32 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
S43 Phosphorylation
S56 Phosphorylation
T129 Phosphorylation
S176 Phosphorylation
T250 Phosphorylation
S273 Phosphorylation
S564 Phosphorylation
S567 Phosphorylation

Research Backgrounds

Function:

Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. May serve to import iron into the mitochondria.

PTMs:

Ubiquitinated by WWP2.

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Early endosome.

Endosome membrane>Multi-pass membrane protein. Mitochondrion outer membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Also found in extracellular vesicles different from exosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.

Subunit Structure:

Forms a complex with NDFIP1 and NEDD4L, in cortical neurons, in response to iron and colbalt exposure; this interaction leads to ubiquitination by NEDD4L and proteasome-dependent degradation. Interacts with NDFIP2. Interacts with COX2 and TOM6 at the outer mitochondrion membrane. Interacts with ARRDC1; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism. Interacts with ARRDC4; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism (By similarity).

Family&Domains:

Belongs to the NRAMP family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells. Oxidative medicine and cellular longevity, 2021 (PubMed: 34707773)

Application: WB    Species: Human    Sample: OS cells

Figure 2 Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μM, 20 μM, and 40 μM) for 24 h. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and #p < 0.001 vs. the control group.

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