SESN3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells, using SESN3 Antibody.

Western blot analysis of extracts from COS-7, using SESN3 Antibody.

Product:	SESN3 Antibody
Catalog:	DF12735
Description:	Rabbit polyclonal antibody to SESN3
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	57 kDa; 57kD(Calculated).
Uniprot:	P58005
RRID:	AB_2845696

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

SESN3 Antibody detects endogenous levels of total SESN3.

RRID:

AB_2845696
Cite Format: Affinity Biosciences Cat# DF12735, RRID:AB_2845696.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC29667; OTTHUMP00000236979; OTTHUMP00000236986; SESN3; SESN3_HUMAN; SEST3; Sestrin 3; Sestrin-3;

Immunogens

Immunogen:

Uniprot:

P58005(SESN3_HUMAN) >>Visit HPA database.

Gene(ID):

SESN3(143686)

Expression:

P58005 SESN3_HUMAN:

Widely expressed.

Sequence:

P58005 SESN3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSDFEDDMIITSDVSRYIEDPGFGYEDFARRGEEHLPTFRAQDYTWENHGFSLVNRLYSDIGHLLDEKFRMVYNLTYNTMATHEDVDTTMLRRALFNYVHCMFGIRYDDYDYGEVNQLLERSLKVYIKTVTCYPERTTKRMYDSYWRQFKHSEKVHVNLLLMEARMQAELLYALRAITRHLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P58005 As Substrate

P58005

Site	PTM Type	Source
R3	Methylation	Uniprot
S7	Phosphorylation	Uniprot
K47	Ubiquitination	Uniprot
R98	Methylation	Uniprot
K153	Ubiquitination	Uniprot
K171	Ubiquitination	Uniprot
K176	Ubiquitination	Uniprot
S282	Phosphorylation	Uniprot
S287	Phosphorylation	Uniprot
T288	Phosphorylation	Uniprot
K378	Ubiquitination	Uniprot
K438	Ubiquitination	Uniprot
T447	Phosphorylation	Uniprot
S454	Phosphorylation	Uniprot
Y455	Phosphorylation	Uniprot

Research Backgrounds

P58005_SESN3_HUMAN

Function:

May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway. May also regulate the insulin-receptor signaling pathway through activation of TORC2 (By similarity). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity).

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with the GATOR2 complex which is composed of MIOS, SEC13, SEH1L, WDR24 and WDR59; the interaction is not regulated by leucine. Interacts with RRAGA, RRAGB, RRAGC and RRAGD; may function as a guanine nucleotide dissociation inhibitor for RRAGs and regulate them. Interacts with the TORC2 complex; through RICTOR (By similarity).

Family&Domains:

The C-terminal domain may mediate interaction with GATOR2 and regulation of TORC1 signaling.

Belongs to the sestrin family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

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SESN3 Antibody - #DF12735