RSPO3 Antibody - #DF12728

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Product: RSPO3 Antibody
Catalog: DF12728
Description: Rabbit polyclonal antibody to RSPO3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 40 kDa; 31kD(Calculated).
Uniprot: Q9BXY4
RRID: AB_2845689

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
RSPO3 Antibody detects endogenous levels of total RSPO3.
RRID:
AB_2845689
Cite Format: Affinity Biosciences Cat# DF12728, RRID:AB_2845689.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CRISTIN1; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; R-spondin 3 homolog; R-spondin-3 [Precursor]; R-spondin-3; Roof plate specific spondin-3; Roof plate-specific spondin-3; RSPO3; RSPO3_HUMAN; Thrombospondin type I domain containing 2; Thrombospondin type-1 domain-containing protein 2; THSD2;

Immunogens

Immunogen:
Uniprot:

Q9BXY4(RSPO3_HUMAN) >>Visit HPA database.

Gene(ID):
RSPO3(84870)
Expression:
Q9BXY4 RSPO3_HUMAN:

Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node (PubMed:12463421). Highly expressed in endothelial cells (PubMed:26766444).

Sequence:
MHLRLISWLFIILNFMEYIGSQNASRGRRQRRMHPNVSQGCQGGCATCSDYNGCLSCKPRLFFALERIGMKQIGVCLSSCPSGYYGTRYPDINKCTKCKADCDTCFNKNFCTKCKSGFYLHLGKCLDNCPEGLEANNHTMECVSIVHCEVSEWNPWSPCTKKGKTCGFKRGTETRVREIIQHPSAKGNLCPPTNETRKCTVQRKKCQKGERGKKGRERKRKKPNKGESKEAIPDSKSLESSKEIPEQRENKQQQKKRKVQDKQKSVSVSTVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
70
Xenopus
60
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXY4 As Substrate

Site PTM Type Enzyme
S235 Phosphorylation
S237 Phosphorylation
S240 Phosphorylation
S241 Phosphorylation

Research Backgrounds

Function:

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (By similarity). Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. Highly expressed in endothelial cells.

Subunit Structure:

Interacts with the extracellular domain of FZD8 and LRP6 (By similarity). It however does not form a ternary complex with FZD8 and LRP6 (By similarity). Interacts with WNT1 (By similarity). Binds heparin. Interacts with LGR4, LGR5 and LGR6.

Family&Domains:

The FU repeats are required for activation and stabilization of beta-catenin.

Belongs to the R-spondin family.

References

1). Differential Gene Expression Profiling in Alveolar Echinococcosis Identifies Potential Biomarkers Associated With Angiogenesis. Open Forum Infectious Diseases, 2023 (PubMed: 36817746) [IF=4.2]

Application: IHC    Species: Human    Sample: AE samples

Figure 5. Immunohistochemical analysis of CD34, vascular endothelial growth factor (VEGF), and angiogenesis-related differentially expressed genes in the experimental (alveolar echinococcosis [AE]) and control (AE control group [AEC]) group samples. (A) CD34 and VEGF staining in AE samples showed different degrees of vascularization. Increased expression of SPP1, RSPO3, APLN, TWIST1, ADAM12, and FOXC2 was observed in the AE samples, whereas low or no expression of these genes was observed in the AEC samples. (B) The H-scores of CD34, VEGF, SPP1, RSPO3, APLN, TWIST1, ADAM12, and FOXC2 were significantly higher in the AE samples than in the AEC samples (values represent the mean ± standard error of the mean: ∗∗∗P < .001, ∗∗∗∗P < .0001).

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