Ribophorin I Antibody - #DF12727

(3)  
Product: Ribophorin I Antibody
Catalog: DF12727
Description: Rabbit polyclonal antibody to Ribophorin I
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 69 kDa; 69kD(Calculated).
Uniprot: P04843
RRID: AB_2845688

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Rabbit(92%), Dog(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Ribophorin I Antibody detects endogenous levels of total Ribophorin I.
RRID:
AB_2845688
Cite Format: Affinity Biosciences Cat# DF12727, RRID:AB_2845688.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D6Wsu137e; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; EC 2.4.1.119; Oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; Ribophorin-1; RPN-I; Rpn1; RPN1_HUMAN;

Immunogens

Immunogen:
Uniprot:

P04843(RPN1_HUMAN) >>Visit HPA database.

Gene(ID):
RPN1(6184)
Expression:
P04843 RPN1_HUMAN:

Expressed in all tissues tested.

Sequence:
MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRIGLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Dog
92
Rabbit
92
Xenopus
83
Zebrafish
75
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04843 As Substrate

Site PTM Type Enzyme
K37 Ubiquitination
T39 Phosphorylation
K48 Ubiquitination
K89 Ubiquitination
K116 Ubiquitination
Y135 Phosphorylation
T136 Phosphorylation
Y161 Phosphorylation
S164 Phosphorylation
K187 Acetylation
K187 Ubiquitination
S194 Phosphorylation
Y200 Phosphorylation
Y209 Phosphorylation
K215 Ubiquitination
S222 Phosphorylation
K258 Ubiquitination
Y264 Phosphorylation
Y266 Phosphorylation
K281 Ubiquitination
Y292 Phosphorylation
Y293 Phosphorylation
N299 N-Glycosylation
K352 Ubiquitination
K371 Ubiquitination
K379 Ubiquitination
S385 Phosphorylation
Y387 Phosphorylation
S390 Phosphorylation
Y398 Phosphorylation
T399 Phosphorylation
K413 Acetylation
K413 Ubiquitination
K414 Acetylation
K414 Ubiquitination
K465 Ubiquitination
K474 Ubiquitination
C477 S-Nitrosylation
K488 Ubiquitination
S510 Phosphorylation
S514 Phosphorylation
K516 Acetylation
K516 Ubiquitination
K517 Ubiquitination
S518 Phosphorylation
K524 Acetylation
K524 Ubiquitination
S528 Phosphorylation
S535 Phosphorylation
K538 Acetylation
K538 Sumoylation
K538 Ubiquitination
S542 Phosphorylation
R547 Methylation
K553 Acetylation
K553 Ubiquitination
K559 Ubiquitination
K564 Ubiquitination
K576 Ubiquitination
K578 Ubiquitination
K579 Ubiquitination
K587 Acetylation
K587 Ubiquitination
T598 Phosphorylation
K599 Ubiquitination

Research Backgrounds

Function:

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Subcellular Location:

Endoplasmic reticulum. Endoplasmic reticulum membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested.

Subunit Structure:

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).

Family&Domains:

Belongs to the OST1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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