RMI1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells, using RMI1 Antibody.

Product:	RMI1 Antibody
Catalog:	DF12724
Description:	Rabbit polyclonal antibody to RMI1
Application:	WB
Reactivity:	Human
Mol.Wt.:	75 kDa; 70kD(Calculated).
Uniprot:	Q9H9A7
RRID:	AB_2845685

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

RMI1 Antibody detects endogenous levels of total RMI1.

RRID:

AB_2845685
Cite Format: Affinity Biosciences Cat# DF12724, RRID:AB_2845685.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BLAP75; BLM associated polypeptide 75 kDa; BLM associated protein 75 kDa; BLM-associated protein of 75 kDa; C9orf76; FAAP75; FLJ12888; Homolog of yeast RecQ mediated genome instability 1; RecQ mediated genome instability 1; RecQ-mediated genome instability protein 1; Rmi1; RMI1 homolog; RMI1 RecQ mediated genome instability 1 homolog; RMI1_HUMAN; RP11-346I8;

Immunogens

Immunogen:

Uniprot:

Q9H9A7(RMI1_HUMAN) >>Visit HPA database.

Gene(ID):

RMI1(80010)

Sequence:

Q9H9A7 RMI1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNVTSIALRAETWLLAAWHVKVPPMWLEACINWIQEENNNVNLSQAQMNKQVFEQWLLTDLRDLEHPLLPDGILEIPKGELNGFYALQINSLVDVSQPAYSQIQKLRGKNTTNDLVTAEAQVTPKPWEAKPSRMLMLQLTDGIVQIQGMEYQPIPILHSDLPPGTKILIYGNISFRLGVLLLKPENVKVLGGEVDALLEEYAQEKVLARLIGEPDLVVSVIPNNSNENIPRVTDVLDPALGPSDEELLASLDENDELTANNDTSSERCFTTGSSSNTIPTRQSSFEPEFVISPRPKEEPSNLSIHVMDGELDDFSLEEALLLEETVQKEQMETKELQPLTFNRNADRSIERFSHNPNTTNNFSLTCKNGNNNWSEKNVSEQMTNEDKSFGCPSVRDQNRSIFSVHCNVPLAHDFTNKEKNLETDNKIKQTSSSDSHSLNNKILNREVVNYVQKRNSQISNENDCNLQSCSLRSSENSINLSIAMDLYSPPFVYLSVLMASKPKEVTTVKVKAFIVTLTGNLSSSGGIWSITAKVSDGTAYLDVDFVDEILTSLIGFSVPEMKQSKKDPLQYQKFLEGLQKCQRDLIDLCCLMTISFNPSLSKAMVLALQDVNMEHLENLKKRLNK

PTMs - Q9H9A7 As Substrate

Q9H9A7

Site	PTM Type	Source
M1	Acetylation	Uniprot
T4	Phosphorylation	Uniprot
K109	Ubiquitination	Uniprot
T111	Phosphorylation	Uniprot
T112	Phosphorylation	Uniprot
T123	Phosphorylation	Uniprot
K125	Ubiquitination	Uniprot
K130	Ubiquitination	Uniprot
S132	Phosphorylation	Uniprot
K205	Ubiquitination	Uniprot
S219	Phosphorylation	Uniprot
S225	Phosphorylation	Uniprot
S250	Phosphorylation	Uniprot
T277	Phosphorylation	Uniprot
T280	Phosphorylation	Uniprot
S283	Phosphorylation	Uniprot
S284	Phosphorylation	Uniprot
S292	Phosphorylation	Uniprot
K334	Sumoylation	Uniprot
K334	Ubiquitination	Uniprot
T359	Phosphorylation	Uniprot
S363	Phosphorylation	Uniprot
K376	Ubiquitination	Uniprot
T383	Phosphorylation	Uniprot
K387	Ubiquitination	Uniprot
S388	Phosphorylation	Uniprot
S400	Phosphorylation	Uniprot
S403	Phosphorylation	Uniprot
T415	Phosphorylation	Uniprot
K428	Ubiquitination	Uniprot
K441	Ubiquitination	Uniprot
Y450	Phosphorylation	Uniprot
K453	Ubiquitination	Uniprot
S456	Phosphorylation	Uniprot
S524	Phosphorylation	Uniprot
K566	Ubiquitination	Uniprot
K573	Ubiquitination	Uniprot
K580	Ubiquitination	Uniprot

Research Backgrounds

Q9H9A7_RMI1_HUMAN

Function:

Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.

Subcellular Location:

Nucleus.
Note: Forms foci in response to DNA damage.

Subunit Structure:

Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM. Directly interacts with RMI2 and TOP3A. May bind DHJ. Interacts (via N-terminal region) with BLM; the interaction is direct.

Family&Domains:

Belongs to the RMI1 family.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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RMI1 Antibody - #DF12724