QKI Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from K-562 cells, using QKI Antibody.

Western blot analysis of extracts from Rat liver, using QKI Antibody.

DF12715 staining Hela cells by IF/ICC.

Fig.

Product:	QKI Antibody
Catalog:	DF12715
Description:	Rabbit polyclonal antibody to QKI
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	38 kDa; 38kD(Calculated).
Uniprot:	Q96PU8
RRID:	AB_2845676

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

QKI Antibody detects endogenous levels of total QKI.

RRID:

AB_2845676
Cite Format: Affinity Biosciences Cat# DF12715, RRID:AB_2845676.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp586I0923; HKQ; Homolog of mouse quaking QKI KH domain RNA binding protein; Hqk; HQK1; HqkI; OTTHUMP00000017581; OTTHUMP00000017582; OTTHUMP00000017583; Protein quaking; QK; QK1; QK3; QKI; QKI_HUMAN; QKI1; Quaking homolog; Quaking homolog KH domain RNA binding; Quaking homolog KH domain RNA binding mouse; Quaking isoform 1; Quaking protein; RNA binding protein HQK;

Immunogens

Immunogen:

Uniprot:

Q96PU8(QKI_HUMAN) >>Visit HPA database.

Gene(ID):

QKI(9444)

Expression:

Q96PU8 QKI_HUMAN:

Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.

Sequence:

Q96PU8 QKI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96PU8 As Substrate

Q96PU8

Site	PTM Type	Source
K10	Ubiquitination	Uniprot
K25	Ubiquitination	Uniprot
K26	Ubiquitination	Uniprot
T60	Phosphorylation	Uniprot
S64	Phosphorylation	Uniprot
T65	Phosphorylation	Uniprot
K67	Ubiquitination	Uniprot
S69	Phosphorylation	Uniprot
K91	Ubiquitination	Uniprot
K111	Ubiquitination	Uniprot
S188	Phosphorylation	Uniprot
K190	Ubiquitination	Uniprot
T203	Phosphorylation	Uniprot
K210	Ubiquitination	Uniprot
S211	Phosphorylation	Uniprot
R227	Methylation	Uniprot
R242	Methylation	Uniprot
T243	Phosphorylation	Uniprot
T245	Phosphorylation	Uniprot
R256	Methylation	Uniprot
R336	Methylation	Uniprot

Research Backgrounds

Q96PU8_QKI_HUMAN

Function:

RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability. In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor (By similarity).

PTMs:

Methylated by PRMT1.

Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.

Tissue Specificity:

Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.

Subunit Structure:

Homodimer. Does not require RNA to homodimerize. Able to heterodimerize with BICC1 (By similarity).

Family&Domains:

The KH domain and the Qua2 region are involved in RNA binding.

References

1). YY1 complex promotes Quaking expression via super-enhancer binding during EMT of hepatocellular carcinoma. CANCER RESEARCH, 2019 (PubMed: 30760518) [IF=11.2]

Application: WB Species: human Sample: PLC-PRF-5 cells

Fig. 2 |YY1 enhances QKI gene transcription and translation through the formation of a complex with p65 and p300. (g) QKI expression measured through Western blot analysis in PLC-PRF-5 cells transfected with YY1 or p65 alone or co-transfected with QKI siRNA.

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For Research Use Only.
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QKI Antibody - #DF12715