PSMC1 Antibody - #DF12710

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Product: PSMC1 Antibody
Catalog: DF12710
Description: Rabbit polyclonal antibody to PSMC1
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 57 kDa; 49kD(Calculated).
Uniprot: P62191
RRID: AB_2845671

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PSMC1 Antibody detects endogenous levels of total PSMC1.
RRID:
AB_2845671
Cite Format: Affinity Biosciences Cat# DF12710, RRID:AB_2845671.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

26S protease regulatory subunit 4; 26S proteasome AAA-ATPase subunit RPT2; MGC24583; MGC8541; P26s4; p56; protease 26S, subunit 4; Proteasome (prosome macropain) 26S subunit ATPase 1; Proteasome 26S ATPase subunit 1; Proteasome 26S subunit ATPase 1; PRS4_HUMAN; PSMC 1; Psmc1; S4;

Immunogens

Immunogen:
Uniprot:

P62191(PRS4_HUMAN) >>Visit HPA database.

Gene(ID):
PSMC1(5700)
Sequence:
MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62191 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S4 Phosphorylation
S6 Phosphorylation
K24 Acetylation
K24 Ubiquitination
Y25 Phosphorylation
K48 Ubiquitination
T53 Phosphorylation
T56 Phosphorylation
C58 S-Nitrosylation
K61 Ubiquitination
K64 Ubiquitination
K69 Ubiquitination
Y71 Phosphorylation
K86 Ubiquitination
K91 Ubiquitination
K98 Sumoylation
K98 Ubiquitination
K155 Ubiquitination
K178 Ubiquitination
K198 Ubiquitination
Y210 Phosphorylation
K217 Ubiquitination
K220 Ubiquitination
Y225 Phosphorylation
K232 Ubiquitination
K237 Ubiquitination
K258 Acetylation
K258 Ubiquitination
K265 Acetylation
K265 Ubiquitination
K293 Ubiquitination
S297 Phosphorylation
T308 Phosphorylation
R322 Methylation
K326 Ubiquitination
K350 Ubiquitination
K359 Ubiquitination
S370 Phosphorylation
K387 Ubiquitination
S391 Phosphorylation
K396 Ubiquitination
K413 Ubiquitination
K420 Ubiquitination
K423 Ubiquitination
K429 Acetylation
K429 Ubiquitination
K430 Sumoylation
K430 Ubiquitination
T434 Phosphorylation
Y439 Phosphorylation

Research Backgrounds

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Subcellular Location:

Cytoplasm. Nucleus. Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC1 and few additional components. Interacts with SCA7. Interacts with NGLY1. Interacts with PAAF1.

Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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