PRUNE2 Antibody - #DF12709
(3)
| Product: | PRUNE2 Antibody |
| Catalog: | DF12709 |
| Description: | Rabbit polyclonal antibody to PRUNE2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Horse, Sheep, Rabbit, Chicken |
| Mol.Wt.: | 150 kDa; 341kD(Calculated). |
| Uniprot: | Q8WUY3 |
| RRID: | AB_2845670 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PRUNE2 Antibody detects endogenous levels of total PRUNE2.
RRID:
AB_2845670
Cite Format: Affinity Biosciences Cat# DF12709, RRID:AB_2845670.
Cite Format: Affinity Biosciences Cat# DF12709, RRID:AB_2845670.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
A214N16 3; BCH motif containing molecule at the carboxyl terminal region 1; BMCC1; BNIP2 motif containing molecule at the carboxyl terminal region 1; BNIPXL; C9orf65; chromosome 9 open reading frame 65; prune homolog 2 (Drosophila); Prune2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8WUY3 PRUN2_HUMAN:
A high level of expression seen in the nervous system (brain, cerebellum and spinal cord) as well as adrenal gland. Expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. Expression is significantly higher in favorable tumors than aggressive ones.
Sequence:
- Q8WUY3 PRUN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLENCLFHSNITSDLKAFTDKFGFDVLILFSSYLSEEQQPRRQIAVYSENMELCSQICCELEECQNPCLELEPFDCGCDEILVYQQEDPSVTCDQVVLVVKEVINRRCPEMVSNSRTSSTEAVAGSAPLSQGSSGIMELYGSDIEPQPSSVNFIENPPDLNDSNQAQVDANVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVGEGAGPHHTLLPGLDSYSPIPEGAVAEEHAWSGEHGEHFDLFNFDPAPMASGQSQQSSHSADYSPADDFFPNSDLSEGQLPAGPEGLDGMGTNMSNYSSSSLLSGAGKDSLVEHDEEFVQRQDSPRDNSERNLSLTDFVGDESPSPERLKNTGKRIPPTPMNSLVESSPSTEEPASLYTEDMTQKATDTGHMGPPQTHARCSSWWGGLEIDSKNIADAWSSSEQESVFQSPESWKEHKPSSIDRRASDSVFQPKSLEFTKSGPWESEFGQPELGSNDIQDKNEESLPFQNLPMEKSPLPNTSPQGTNHLIEDFASLWHSGRSPTAMPEPWGNPTDDGEPAAVAPFPAWSAFGKEDHDEALKNTWNLHPTSSKTPSVRDPNEWAMAKSGFAFSSSELLDNSPSEINNEAAPEIWGKKNNDSRDHIFAPGNPSSDLDHTWTNSKPPKEDQNGLVDPKTRGKVYEKVDSWNLFEENMKKGGSDVLVPWEDSFLSYKCSDYSASNLGEDSVPSPLDTNYSTSDSYTSPTFAGDEKETEHKPFAKEEGFESKDGNSTAEETDIPPQSLQQSSRNRISSGPGNLDMWASPHTDNSSEINTTHNLDENELKTEHTDGKNISMEDDVGESSQSSYDDPSMMQLYNETNRQLTLLHSSTNSRQTAPDSLDLWNRVILEDTQSTATISDMDNDLDWDDCSGGAAIPSDGQTEGYMAEGSEPETRFTVRQLEPWGLEYQEANQVDWELPASDEHTKDSAPSEHHTLNEKSGQLIANSIWDSVMRDKDMSSFMLPGSSHITDSEQRELPPEIPSHSANVKDTHSPDAPAASGTSESEALISHLDKQDTERETLQSDAASLATRLENPGYFPHPDPWKGHGDGQSESEKEAQGATDRGHLDEEEVIASGVENASGISEKGQSDQELSSLVASEHQEICIKSGKISSLAVTFSPQTEEPEEVLEYEEGSYNLDSRDVQTGMSADNLQPKDTHEKHLMSQRNSGETTETSDGMNFTKYVSVPEKDLEKTEECNFLEPENVGGGPPHRVPRSLDFGDVPIDSDVHVSSTCSEITKNLDVKGSENSLPGAGSSGNFDRDTISSEYTHSSASSPELNDSSVALSSWGQQPSSGYQEENQGNWSEQNHQESELITTDGQVEIVTKVKDLEKNRINEFEKSFDRKTPTFLEIWNDSVDGDSFSSLSSPETGKYSEHSGTHQESNLIASYQEKNEHDISATVQPEDARVISTSSGSDDDSVGGEESIEEEIQVANCHVAEDESRAWDSLNESNKFLVTADPKSENIYDYLDSSEPAENENKSNPFCDNQQSSPDPWTFSPLTETEMQITAVEKEKRSSPETGTTGDVAWQISPKASFPKNEDNSQLEMLGFSADSTEWWKASPQEGRLIESPFERELSDSSGVLEINSSVHQNASPWGVPVQGDIEPVETHYTNPFSDNHQSPFLEGNGKNSHEQLWNIQPRQPDPDADKFSQLVKLDQIKEKDSREQTFVSAAGDELTPETPTQEQCQDTMLPVCDHPDTAFTHAEENSCVTSNVSTNEGQETNQWEQEKSYLGEMTNSSIATENFPAVSSPTQLIMKPGSEWDGSTPSEDSRGTFVPDILHGNFQEGGQLASAAPDLWIDAKKPFSLKADGENPDILTHCEHDSNSQASDSPDICHDSEAKQETEKHLSACMGPEVESSELCLTEPEIDEEPIYEPGREFVPSNAELDSENATVLPPIGYQADIKGSSQPASHKGSPEPSEINGDNSTGLQVSEKGASPDMAPILEPVDRRIPRIENVATSIFVTHQEPTPEGDGSWISDSFSPESQPGARALFDGDPHLSTENPALVPDALLASDTCLDISEAAFDHSFSDASGLNTSTGTIDDMSKLTLSEGHPETPVDGDLGKQDICSSEASWGDFEYDVMGQNIDEDLLREPEHFLYGGDPPLEEDSLKQSLAPYTPPFDLSYLTEPAQSAETIEEAGSPEDESLGCRAAEIVLSALPDRRSEGNQAETKNRLPGSQLAVLHIREDPESVYLPVGAGSNILSPSNVDWEVETDNSDLPAGGDIGPPNGASKEISELEEEKTIPTKEPEQIKSEYKEERCTEKNEDRHALHMDYILVNREENSHSKPETCEERESIAELELYVGSKETGLQGTQLASFPDTCQPASLNERKGLSAEKMSSKSDTRSSFESPAQDQSWMFLGHSEVGDPSLDARDSGPGWSGKTVEPFSELGLGEGPQLQILEEMKPLESLALEEASGPVSQSQKSKSRGRAGPDAVTLQAVTHDNEWEMLSPQPVQKNMIPDTEMEEETEFLELGTRISRPNGLLSEDVGMDIPFEEGVLSPSAADMRPEPPNSLDLNDTHPRRIKLTAPNINLSLDQSEGSILSDDNLDSPDEIDINVDELDTPDEADSFEYTGHDPTANKDSGQESESIPEYTAEEEREDNRLWRTVVIGEQEQRIDMKVIEPYRRVISHGGYYGDGLNAIIVFAACFLPDSSRADYHYVMENLFLYVISTLELMVAEDYMIVYLNGATPRRRMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILAVTRPFISSKFSSKIKYVNSLSELSGLIPMDCIHIPESIIKLDEELREASEAAKTSCLYNDPEMSSMEKDIDLKLKEKP
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Sheep
100
Chicken
100
Rabbit
100
Pig
0
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8WUY3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| T588 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S692 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S737 | Phosphorylation | Uniprot | |
| S918 | Phosphorylation | Uniprot | |
| S1024 | Phosphorylation | Uniprot | |
| S1211 | Phosphorylation | Uniprot | |
| T1262 | Phosphorylation | Uniprot | |
| S1264 | Phosphorylation | Uniprot | |
| S1420 | Phosphorylation | Uniprot | |
| S1440 | Phosphorylation | Uniprot | |
| S1613 | Phosphorylation | Uniprot | |
| S1670 | Phosphorylation | Uniprot | |
| S1719 | Phosphorylation | Uniprot | |
| S1723 | Phosphorylation | Uniprot | |
| Y1738 | Phosphorylation | Uniprot | |
| Y1740 | Phosphorylation | Uniprot | |
| S1744 | Phosphorylation | Uniprot | |
| S1789 | Phosphorylation | Uniprot | |
| T1792 | Phosphorylation | Uniprot | |
| T1795 | Phosphorylation | Uniprot | |
| S1803 | Phosphorylation | Uniprot | |
| S1842 | Phosphorylation | Uniprot | |
| S1849 | Phosphorylation | Uniprot | |
| T2091 | Phosphorylation | Uniprot | |
| Y2147 | Phosphorylation | Uniprot | |
| S2189 | Phosphorylation | Uniprot | |
| S2193 | Phosphorylation | Uniprot | |
| S2200 | Phosphorylation | Uniprot | |
| T2201 | Phosphorylation | Uniprot | |
| S2211 | Phosphorylation | Uniprot | |
| S2325 | Phosphorylation | Uniprot | |
| T2331 | Phosphorylation | Uniprot | |
| S2384 | Phosphorylation | Uniprot | |
| S2439 | Phosphorylation | Uniprot | |
| S2453 | Phosphorylation | Uniprot | |
| Y2550 | Phosphorylation | Uniprot | |
| S2866 | Phosphorylation | Uniprot | |
| Y2945 | Phosphorylation | Uniprot | |
| Y2958 | Phosphorylation | Uniprot | |
| T2967 | Phosphorylation | Uniprot | |
| K2980 | Methylation | Uniprot |
Research Backgrounds
Function:
May play an important role in regulating differentiation, survival and aggressiveness of the tumor cells.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
A high level of expression seen in the nervous system (brain, cerebellum and spinal cord) as well as adrenal gland. Expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. Expression is significantly higher in favorable tumors than aggressive ones.
Family&Domains:
Belongs to the PPase class C family. Prune subfamily.
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