PRPF4 Antibody - #DF12707

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Product: PRPF4 Antibody
Catalog: DF12707
Description: Rabbit polyclonal antibody to PRPF4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 58 kDa; 58kD(Calculated).
Uniprot: O43172
RRID: AB_2845668

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
PRPF4 Antibody detects endogenous levels of total PRPF4.
RRID:
AB_2845668
Cite Format: Affinity Biosciences Cat# DF12707, RRID:AB_2845668.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hPrp4; HPRP4P; PRP4; PRP4 homolog; PRP4 pre mRNA processing factor 4 homolog (yeast); PRP4/STK/WD splicing factor; PRP4_HUMAN; Prp4p; PRPF4; RP70; SNRNP60; U4/U6 small nuclear ribonucleoprotein Prp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4;

Immunogens

Immunogen:
Uniprot:

O43172(PRP4_HUMAN) >>Visit HPA database.

Gene(ID):
PRPF4(9128)
Sequence:
MASSRASSTQATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWIANYSLPRAMKRLEEARLHKEIPETTRTSQMQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Zebrafish
83
Xenopus
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43172 As Substrate

Site PTM Type Enzyme
K15 Sumoylation
K27 Acetylation
K27 Sumoylation
K27 Ubiquitination
Y31 Phosphorylation
Y32 Phosphorylation
S34 Phosphorylation
K38 Ubiquitination
K45 Sumoylation
K45 Ubiquitination
K53 Acetylation
K53 Ubiquitination
K146 Acetylation
K147 Acetylation
K159 Sumoylation
K159 Ubiquitination
Y162 Phosphorylation
K200 Ubiquitination
K216 Ubiquitination
S240 Phosphorylation
K284 Ubiquitination
K292 Ubiquitination
R345 Methylation
K456 Acetylation
S483 Phosphorylation
K486 Ubiquitination

Research Backgrounds

Function:

Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).

Subcellular Location:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Interacts directly with PRPF18, PPIH and PRPF3. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Interacts with ERCC6.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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