PI4KA Antibody - #DF12696

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Product: PI4KA Antibody
Catalog: DF12696
Description: Rabbit polyclonal antibody to PI4KA
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 231 kDa; 237kD(Calculated).
Uniprot: P42356
RRID: AB_2845657

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
PI4KA Antibody detects endogenous levels of total PI4KA.
RRID:
AB_2845657
Cite Format: Affinity Biosciences Cat# DF12696, RRID:AB_2845657.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.1.67; Phosphatidylinositol 4 kinase alpha; Phosphatidylinositol 4 kinase catalytic alpha polypeptide; Phosphatidylinositol 4-kinase alpha; PI4 kinase; PI4-kinase alpha; PI4K ALPHA; PI4K-alpha; pi4K230; PI4KA; PI4KA_HUMAN; PIK4; PIK4CA; PtdIns 4 kinase; PtdIns-4-kinase alpha;

Immunogens

Immunogen:
Uniprot:

P42356(PI4KA_HUMAN) >>Visit HPA database.

Gene(ID):
PI4KA(5297)
Expression:
P42356 PI4KA_HUMAN:

Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes.

Sequence:
MAAAPARGGGGGGGGGGGCSGSGSSASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARKGRGALPVAESFSFCLVTLLSDVAYRDPSLRDEILEVLLQVLHVLLGMCQALEIQDKEYLCKYAIPCLIGISRAFGRYSNMEESLLSKLFPKIPPHSLRVLEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSVSPLFNGVTYKEFNIPLEMLRELLNLVKKIVEEAVLKSLDAIVASVMEANPSADLYYTSFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNTSQGELQKILHDADRIHNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGNDIKISVTNEHSESTLNVMSGKKSQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLSERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMVQDLHSALDRSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42356 As Substrate

Site PTM Type Enzyme
R7 Methylation
Y197 Phosphorylation
S198 Phosphorylation
S203 Phosphorylation
K207 Ubiquitination
S230 Phosphorylation
S236 Phosphorylation
S240 Phosphorylation
T250 Phosphorylation
K252 Ubiquitination
K254 Ubiquitination
T255 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
S259 Phosphorylation
S260 Phosphorylation
S262 Phosphorylation
S265 Phosphorylation
K416 Ubiquitination
R423 Methylation
S429 Phosphorylation
K432 Ubiquitination
K461 Ubiquitination
K465 Ubiquitination
S502 Phosphorylation
T504 Phosphorylation
S506 Phosphorylation
K523 Ubiquitination
Y524 Phosphorylation
S526 Phosphorylation
Y528 Phosphorylation
T530 Phosphorylation
T548 Phosphorylation
K555 Ubiquitination
K556 Ubiquitination
K607 Ubiquitination
K632 Ubiquitination
Y691 Phosphorylation
T694 Phosphorylation
K695 Ubiquitination
K759 Ubiquitination
S763 Phosphorylation
K785 Ubiquitination
K838 Ubiquitination
Y847 Phosphorylation
K852 Ubiquitination
Y901 Phosphorylation
S907 Phosphorylation
T908 Phosphorylation
Y919 Phosphorylation
K988 Acetylation
Y1031 Phosphorylation
Y1032 Phosphorylation
K1055 Ubiquitination
K1070 Ubiquitination
K1077 Ubiquitination
K1133 Ubiquitination
K1134 Ubiquitination
Y1154 Phosphorylation
R1158 Methylation
K1171 Ubiquitination
K1336 Ubiquitination
S1358 Phosphorylation
T1369 Phosphorylation
S1436 Phosphorylation
S1444 Phosphorylation
T1455 Phosphorylation
K1467 Ubiquitination
K1531 Ubiquitination
K1538 Ubiquitination
K1563 Ubiquitination
S1584 Phosphorylation
Y1663 Phosphorylation
Y1672 Phosphorylation
S1678 Phosphorylation
K1749 Ubiquitination
K1753 Ubiquitination
K1759 Ubiquitination
Y1789 Phosphorylation
K1800 Ubiquitination
Y1803 Phosphorylation
K1806 Ubiquitination
K1819 Ubiquitination
S1828 Phosphorylation
Y1884 Phosphorylation
S2087 Phosphorylation
T2089 Phosphorylation
Y2090 Phosphorylation

Research Backgrounds

Function:

Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Localization to the plasma membrane is mediated by the PI4K complex and association with EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B) (PubMed:23229899). Localization to the plasma membrane is regulated by TMEM150A (PubMed:25608530).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes.

Subunit Structure:

Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B). Interacts with TMEM150A; regulating recruitment to the plasma membrane.

Family&Domains:

Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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