P5CS Antibody - #DF12683

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Product: P5CS Antibody
Catalog: DF12683
Description: Rabbit polyclonal antibody to P5CS
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 87 kDa; 87kD(Calculated).
Uniprot: P54886
RRID: AB_2845645

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(83%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
P5CS Antibody detects endogenous levels of total P5CS.
RRID:
AB_2845645
Cite Format: Affinity Biosciences Cat# DF12683, RRID:AB_2845645.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810433K04Rik; AI429789; Aldehyde dehydrogenase 18 family member A1; Aldehyde dehydrogenase 18A1; Aldehyde dehydrogenase family 18 member A1; ALDH18A1; Delta 1 pyrroline 5 carboxylate synthetase; Delta1 pyrroline 5 carboxlate synthetase; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase; GK; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GPR; GSAS; MGC117316; MGC32233; P5CS; P5CS_HUMAN; PYCS; Pyrroline 5 carboxylate synthetase (glutamate gamma semialdehyde synthetase);

Immunogens

Immunogen:
Uniprot:

P54886(P5CS_HUMAN) >>Visit HPA database.

Gene(ID):
ALDH18A1(5832)
Sequence:
MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAEGRLAAPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVKEAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENLPIPQRNTN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Zebrafish
83
Chicken
83
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54886 As Substrate

Site PTM Type Enzyme
S79 Phosphorylation
S103 Phosphorylation
S117 Phosphorylation
K124 Methylation
S136 Phosphorylation
S143 Phosphorylation
K149 Acetylation
R212 Methylation
K300 Ubiquitination
K347 Acetylation
K347 Ubiquitination
K348 Ubiquitination
K408 Ubiquitination
K409 Acetylation
K424 Ubiquitination
S427 Phosphorylation
S429 Phosphorylation
K432 Ubiquitination
S450 Phosphorylation
T538 Phosphorylation
K550 Ubiquitination
K605 Ubiquitination
C606 S-Nitrosylation
C612 S-Nitrosylation
K643 Ubiquitination
K649 Acetylation
S657 Phosphorylation
S659 Phosphorylation
K662 Ubiquitination
T725 Phosphorylation
R749 Methylation
T759 Phosphorylation
K761 Ubiquitination
K767 Acetylation
K767 Ubiquitination
S772 Phosphorylation
K781 Ubiquitination

Research Backgrounds

Function:

Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.

Subcellular Location:

Mitochondrion inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homohexamer or homotetramer.

Family&Domains:

In the N-terminal section; belongs to the glutamate 5-kinase family.

In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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