NKCC2 Antibody - #DF12671
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| Product: | NKCC2 Antibody |
| Catalog: | DF12671 |
| Description: | Rabbit polyclonal antibody to NKCC2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 135 kDa; 121kD(Calculated). |
| Uniprot: | Q13621 |
| RRID: | AB_2845633 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12671, RRID:AB_2845633.
Fold/Unfold
BSC1; Bumetanide sensitive sodium 3; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney specific Na K Cl symporter; Kidney-specific Na-K-Cl symporter; MGC48843; Na K 2Cl cotransporter; NKCC2; potassiumchloride cotransporter 2; S12A1_HUMAN; Slc12a1; sodium potassium chloride cotransporter 2; solute carrier family 12 (sodium/potassium/chloride transporters); Solute carrier family 12 member 1;
Immunogens
- Q13621 S12A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13621 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y70 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Q9UEW8 (STK39) , O95747 (OXSR1) | Uniprot |
| T100 | Phosphorylation | Q9BYP7 (WNK3) , O95747 (OXSR1) , Q9UEW8 (STK39) | Uniprot |
| T105 | Phosphorylation | O95747 (OXSR1) , Q9BYP7 (WNK3) , Q9UEW8 (STK39) | Uniprot |
| T118 | Phosphorylation | O95747 (OXSR1) | Uniprot |
| S120 | Phosphorylation | O95747 (OXSR1) | Uniprot |
| S122 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S130 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| Y245 | Phosphorylation | Uniprot | |
| K722 | Methylation | Uniprot | |
| K735 | Methylation | Uniprot | |
| Y944 | Phosphorylation | Uniprot | |
| S1056 | Phosphorylation | Uniprot |
Research Backgrounds
Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation. Electrically silent transporter system.
Membrane>Multi-pass membrane protein.
Kidney specific.
When phosphorylated, interacts with PPP3CB.
Belongs to the SLC12A transporter family.
References
Application: WB Species: rat Sample: renal cortex and medulla
Application: WB Species: rat Sample: renal cortex and medulla
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