MCC Antibody | Affinity Biosciences

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Western blot analysis of extracts from C-3 cells, using MCC Antibody.

Product:	MCC Antibody
Catalog:	DF12654
Description:	Rabbit polyclonal antibody to MCC
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	98 kDa; 76kD(Calculated).
Uniprot:	Q8N6Y0
RRID:	AB_2845616

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(83%), Bovine(92%), Horse(83%), Sheep(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

MCC Antibody detects endogenous levels of total MCC.

RRID:

AB_2845616
Cite Format: Affinity Biosciences Cat# DF12654, RRID:AB_2845616.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AIE 75 binding protein; AIE 75 binding protein protein; AIE-75-binding protein; AIEBP; MCC 2; MCC-2; MCC2; Mutated in colon cancer protein 2; USBP1_HUMAN; USH1C binding protein 1; USH1C-binding protein 1; Ushbp1; Usher syndrome 1C binding protein 1; Usher syndrome type 1C protein binding protein 1; Usher syndrome type-1C protein-binding protein 1;

Immunogens

Immunogen:

Uniprot:

Q8N6Y0(USBP1_HUMAN) >>Visit HPA database.

Gene(ID):

USHBP1(83878)

Expression:

Q8N6Y0 USBP1_HUMAN:

Highest level of expression in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.

Sequence:

Q8N6Y0 USBP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSARATRPRSRRGRHAPPGELDPVAESSEEVEAASGSSKPSFAPPPVSSGLEQLGPMEEVSGQGLGSRTDKKMDGGSGRELASAPEVPHKPAVEAHQAPEAALQYKETVPPGNGAPDVFQTLQHTLSSLEAAAAAWRHQPPSHSGPMEFEGTSEGGAGSLGKQEGAGSCQREAARLAERNAWLRLALSSREDELVRTQASLEAIRAEKETLQKEVQELQDSLLRLEPCPHLSHNQAGGSGSGSSSSEADREPWETQDSFSLAHPLLRRLRSHSSTQILGSLPNQPLSPEMHIMEAQMEQLRGSIEKLKCFNRLLSAVLQGYKGRCEGLSMQLGQREAEATALHLALQYSEHCEEAYRVLLALREADSGAGDEAPMSDLQAAEKEAWRLLAQEEAAMDAGAQQNPQPSPEGSSVDKPTPQEVAFQLRSYVQRLQERRSLMKILSEPGPTLAPMPTVPRAEAMVQAILGTQAGPALPRLEKTQIQQDLVAAREALADLMLRLQLVRREKRGLELREAALRALGPAHVLLLEQLRWERAELQAGGANSSGGHSSGGGSSGDEEEWYQGLPAVPGGTSGIDGGQVGRAWDPEKLAQELAASLTRTLDLQEQLQSLRRELEQVAQKGRARRSQSAELNRDLCKAHSALVLAFRGAHRKQEEQRRKLEQQMALMEAQQAEEVAVLEATARALGKPRPPLPPPQLGDTFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Dog

Pig

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N6Y0 As Substrate

Q8N6Y0

Site	PTM Type	Source
S83	Phosphorylation	Uniprot
S315	Phosphorylation	Uniprot
K322	Acetylation	Uniprot
S627	Phosphorylation	Uniprot

Research Backgrounds

Q8N6Y0_USBP1_HUMAN

Tissue Specificity:

Highest level of expression in heart, and moderate to low expression in skeletal muscle, kidney, liver, small intestine, placenta and lung.

Subunit Structure:

Interacts via its C-terminus with the first PDZ domain of USH1C.

Family&Domains:

Belongs to the MCC family.

References

1). Acupoint application inhibits nerve growth factor and attenuates allergic inflammation in allergic rhinitis model rats. Journal of Inflammation-London, 2020 (PubMed: 32063751) [IF=5.1]

Application: IF/ICC Species: rat Sample: mast cells

Fig. 3| Effect of AAT on the expression of NGF、IL-4 and IFN-γ protein in nasal mucosa of AR rats. Note: a Effect of AAT on NGF protein expression in nasal mucosa with Olympus microscope. b The effects of Acupoint Application Therapy on mast cells localization of IL-4 with Olympus microscope.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MCC Antibody - #DF12654