LTBP2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from PC-3 cells, using LTBP2 Antibody.

FIGURE 3 | LTBP2 was highly expressed in lungs of mice with PF.

Product:	LTBP2 Antibody
Catalog:	DF12653
Description:	Rabbit polyclonal antibody to LTBP2
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	195 kDa; 195kD(Calculated).
Uniprot:	Q14767
RRID:	AB_2845615

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%)

Clonality:

Polyclonal

Specificity:

LTBP2 Antibody detects endogenous levels of total LTBP2.

RRID:

AB_2845615
Cite Format: Affinity Biosciences Cat# DF12653, RRID:AB_2845615.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C14orf141; Chromosome 14 open reading frame 141; Latent TGF beta binding protein 2; Latent transforming growth factor beta binding protein 2; Latent-transforming growth factor beta-binding protein 2; LTBP 2; LTBP 3; LTBP-2; Ltbp2; LTBP2_HUMAN; LTBP3; MSTP 031; MSTP031;

Immunogens

Immunogen:

Uniprot:

Q14767(LTBP2_HUMAN) >>Visit HPA database.

Gene(ID):

LTBP2(4053)

Expression:

Q14767 LTBP2_HUMAN:

Expressed in the aorta (at protein level). Expressed in lung, weakly expressed in heart, placenta, liver and skeletal muscle.

Sequence:

Q14767 LTBP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRPRTKARSPGRALRNPWRGFLPLTLALFVGAGHAQRDPVGRYEPAGGDANRLRRPGGSYPAAAAAKVYSLFREQDAPVAGLQPVERAQPGWGSPRRPTEAEARRPSRAQQSRRVQPPAQTRRSTPLGQQQPAPRTRAAPALPRLGTPQRSGAAPPTPPRGRLTGRNVCGGQCCPGWTTANSTNHCIKPVCEPPCQNRGSCSRPQLCVCRSGFRGARCEEVIPDEEFDPQNSRLAPRRWAERSPNLRRSSAAGEGTLARAQPPAPQSPPAPQSPPAGTLSGLSQTHPSQQHVGLSRTVRLHPTATASSQLSSNALPPGPGLEQRDGTQQAVPLEHPSSPWGLNLTEKIKKIKIVFTPTICKQTCARGHCANSCERGDTTTLYSQGGHGHDPKSGFRIYFCQIPCLNGGRCIGRDECWCPANSTGKFCHLPIPQPDREPPGRGSRPRALLEAPLKQSTFTLPLSNQLASVNPSLVKVHIHHPPEASVQIHQVAQVRGGVEEALVENSVETRPPPWLPASPGHSLWDSNNIPARSGEPPRPLPPAAPRPRGLLGRCYLNTVNGQCANPLLELTTQEDCCGSVGAFWGVTLCAPCPPRPASPVIENGQLECPQGYKRLNLTHCQDINECLTLGLCKDAECVNTRGSYLCTCRPGLMLDPSRSRCVSDKAISMLQGLCYRSLGPGTCTLPLAQRITKQICCCSRVGKAWGSECEKCPLPGTEAFREICPAGHGYTYASSDIRLSMRKAEEEELARPPREQGQRSSGALPGPAERQPLRVVTDTWLEAGTIPDKGDSQAGQVTTSVTHAPAWVTGNATTPPMPEQGIAEIQEEQVTPSTDVLVTLSTPGIDRCAAGATNVCGPGTCVNLPDGYRCVCSPGYQLHPSQAYCTDDNECLRDPCKGKGRCINRVGSYSCFCYPGYTLATSGATQECQDINECEQPGVCSGGQCTNTEGSYHCECDQGYIMVRKGHCQDINECRHPGTCPDGRCVNSPGSYTCLACEEGYRGQSGSCVDVNECLTPGVCAHGKCTNLEGSFRCSCEQGYEVTSDEKGCQDVDECASRASCPTGLCLNTEGSFACSACENGYWVNEDGTACEDLDECAFPGVCPSGVCTNTAGSFSCKDCDGGYRPSPLGDSCEDVDECEDPQSSCLGGECKNTVGSYQCLCPQGFQLANGTVCEDVNECMGEEHCAPHGECLNSHGSFFCLCAPGFVSAEGGTSCQDVDECATTDPCVGGHCVNTEGSFNCLCETGFQPSPESGECVDIDECEDYGDPVCGTWKCENSPGSYRCVLGCQPGFHMAPNGDCIDIDECANDTMCGSHGFCDNTDGSFRCLCDQGFEISPSGWDCVDVNECELMLAVCGAALCENVEGSFLCLCASDLEEYDAQEGHCRPRGAGGQSMSEAPTGDHAPAPTRMDCYSGQKGHAPCSSVLGRNTTQAECCCTQGASWGDACDLCPSEDSAEFSEICPSGKGYIPVEGAWTFGQTMYTDADECVIFGPGLCPNGRCLNTVPGYVCLCNPGFHYDASHKKCEDHDECQDLACENGECVNTEGSFHCFCSPPLTLDLSQQRCMNSTSSTEDLPDHDIHMDICWKKVTNDVCSEPLRGHRTTYTECCCQDGEAWSQQCALCPPRSSEVYAQLCNVARIEAEREAGVHFRPGYEYGPGPDDLHYSIYGPDGAPFYNYLGPEDTVPEPAFPNTAGHSADRTPILESPLQPSELQPHYVASHPEPPAGFEGLQAEECGILNGCENGRCVRVREGYTCDCFEGFQLDAAHMACVDVNECDDLNGPAVLCVHGYCENTEGSYRCHCSPGYVAEAGPPHCTAKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Bovine

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14767 As Substrate

Q14767

Site	PTM Type	Source
S59	Phosphorylation	Uniprot
Y60	Phosphorylation	Uniprot
T147	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
S250	Phosphorylation	Uniprot
S372	Phosphorylation	Uniprot
S506	Phosphorylation	Uniprot
T682	Phosphorylation	Uniprot
T731	O-Glycosylation	Uniprot
T1026	Phosphorylation	Uniprot
S1031	Phosphorylation	Uniprot
S1395	Phosphorylation	Uniprot
S1397	O-Glycosylation	Uniprot
T1401	O-Glycosylation	Uniprot
Y1414	Phosphorylation	Uniprot

Research Backgrounds

Q14767_LTBP2_HUMAN

Function:

May play an integral structural role in elastic-fiber architectural organization and/or assembly.

PTMs:

N-Glycosylated.

Contains hydroxylated asparagine residues.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in the aorta (at protein level). Expressed in lung, weakly expressed in heart, placenta, liver and skeletal muscle.

Subunit Structure:

Forms part of the large latent transforming growth factor beta precursor complex; removal is essential for activation of complex. Interacts with SDC4. Interacts (via C-terminal domain) with FBN1 (via N-terminal domain) in a Ca(+2)-dependent manner.

Family&Domains:

Belongs to the LTBP family.

References

1). Elevated latent transforming growth factor beta binding protein 2 in endometriosis promotes endometrial stromal cell invasion and proliferation via the NF-kB signaling pathway. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2022 (PubMed: 35429597) [IF=4.1]

2). Identification of potential pathogenic super-enhancers-driven genes in pulmonary fibrosis. Frontiers in Genetics, 2021 (PubMed: 34054916) [IF=3.7]

Application: WB Species: mouse Sample: lungs

FIGURE 3 | LTBP2 was highly expressed in lungs of mice with PF. (D) Representative bands of western blots showing expression of LTBP2 and β-tubulin in lungs of indicated mice.

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LTBP2 Antibody - #DF12653