LAP3 Antibody - #DF12651

Fig. 2IFN-γ upregulated LAP3 expression in BMECs. a qRT-PCR was used to detect the transcription level of LAP3 in BMECs after treatment with IFN-γ. b ELISA confirmed the content of LAP3 after treatment with IFN-γ in BMECs. c Western blotting was used to test the expression level of LAP3 in BMECs after treatment with 10 ng/mL IFN-γ for 24 h. Full-length blots are presented in Supplementary Fig. S5. d Leucine aminopeptidase activity was used to detect the change of LAP3 enzyme activity in BMECs before and after IFN-γ treatment. e Analysis of intracellular LAP3 expression in BMECs treated with the LAP3 inhibitor, bestatin, for 12 h using qRT-PCR. f HPLC was used to detect the changes of arginine content in BMECs pretreated with 100 mg/mL bestatin for 12 h and stimulated by IFN-γ for 24 h. g Changes in arginine content upon LAP3 overexpression in BMECs with or without IFN-γ treatment, as determined by ELISA. VC, pLenti-CMV-GFP-Puro. pLAP3, pLenti-CMV-LAP3-GFP-Puro. h-i LAP3 expression in normal and malignant BMECs by qRT-PCR (h) and western blotting analysis (i). 0, no IFN-γ treatment, normal BMECs; 30, malignant BMECs after IFN-γ-treated for 30th generations. The relative level of the target protein was estimated using densitometry and the ratio was calculated relative to the GAPDH control. Full-length blots are presented in Supplementary Fig. S6. Bars represent mean values with error bars to represent SD from three independent replicates. Differences between mean values were assessed by two-tailed Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001, compared to control

Fig. 8LAP3 was highly expressed in human breast cancer samples. a, c ELISA experiment was used to detected the protein expression levels of LAP3 (a) and ASS1 (c) in plasma of breast cancer patients and healthy control. Bars represent mean values with error bars to represent SD from three independent replicates. Differences between mean values were assessed by two-tailed Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001, compared to control. b Representative images of IHC staining of LAP3 and ASS1. Scale bar, 200 μm