SSFA2 Antibody - #DF12650

(2)  
Product: SSFA2 Antibody
Catalog: DF12650
Description: Rabbit polyclonal antibody to SSFA2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 180 kDa; 138kD(Calculated).
Uniprot: P28290
RRID: AB_2845612

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%)
Clonality:
Polyclonal
Specificity:
SSFA2 Antibody detects endogenous levels of total SSFA2.
RRID:
AB_2845612
Cite Format: Affinity Biosciences Cat# DF12650, RRID:AB_2845612.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cleavage signal-1 protein; CS-1; CS1; Ki-ras-induced actin-interacting protein; KIAA1927; KRAP; KRAS induced actin interacting protein; SPAG13; Sperm associated antigen 13; Sperm-specific antigen 2; SSFA2; SSFA2_HUMAN;

Immunogens

Immunogen:
Uniprot:

P28290(ITPI2_HUMAN) >>Visit HPA database.

Gene(ID):
SSFA2(6744)
Expression:
P28290 ITPI2_HUMAN:

Strongly expressed in pancreas and testis. Present in colon cancer cells (at protein level).

Sequence:
MDRPLSSSAEAEEELEWQVASRRRKAWAKCRSSWQASETEDLSTEATTQDEEEDEEEDLPGAQLPAAGGRGNVPNEKIAIWLKDCRTPLGASLDEQSSSTLKGVLVRNGGSFEDDLSLGAEANHLHESDAQIENCNNILAKERRLQFHQKGRSMNSTGSGKSSGTVSSVSELLELYEEDPEEILYNLGFGRDEPDIASKIPSRFFNSSSFAKGIDIKVFLSAQMQRMEVENPNYALTSRFRQIEVLTTVANAFSSLYSQVSGTPLQRIGSMSSVTSNKETDPPPPLTRSNTANRLMKTLSKLNLCVDKTEKGESSSPSPSAEKGKILNVSVIEESGNKNDQKSQKIMKKKESSSMLATVKEEVSGSSAAVTENADSDRISDEANSNFNQGTENEQSKETQSHESKLGEESGIVESKLDSDFNISSHSELENSSELKSVHISTPEKEPCAPLTIPSIRNIMTQQKDSFEMEEVQSTEGEAPHVPATYQLGLTKSKRDHLLRTASQHSDSSGFAEDSTDCLSLNHLQVQESLQAMGSSADSCDSETTVTSLGEDLATPTAQDQPYFNESEEESLVPLQKGLEKAAAVADKRKSGSQDFPQCNTIENTGTKQSTCSPGDHIIEITEVEEDLFPAETVELLREASAESDVGKSSESEFTQYTTHHILKSLASIEAKCSDMSSENTTGPPSSMDRVNTALQRAQMKVCSLSNQRMGRSLLKSKDLLKQRYLFAKAGYPLRRSQSLPTTLLSPVRVVSSVNVRLSPGKETRCSPPSFTYKYTPEEEQELEKRVMEHDGQSLVKSTIFISPSSVKKEEAPQSEAPRVEECHHGRTPTCSRLAPPPMSQSTCSLHSIHSEWQERPLCEHTRTLSTHSVPNISGATCSAFASPFGCPYSHRHATYPYRVCSVNPPSAIEMQLRRVLHDIRNSLQNLSQYPMMRGPDPAAAPYSTQKSSVLPLYENTFQELQVMRRSLNLFRTQMMDLELAMLRQQTMVYHHMTEEERFEVDQLQGLRNSVRMELQDLELQLEERLLGLEEQLRAVRMPSPFRSSALMGMCGSRSADNLSCPSPLNVMEPVTELMQEQSYLKSELGLGLGEMGFEIPPGESSESVFSQATSESSSVCSGPSHANRRTGVPSTASVGKSKTPLVARKKVFRASVALTPTAPSRTGSVQTPPDLESSEEVDAAEGAPEVVGPKSEVEEGHGKLPSMPAAEEMHKNVEQDELQQVIREIKESIVGEIRREIVSGLLAAVSSSKASNSKQDYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28290 As Substrate

Site PTM Type Enzyme
K77 Ubiquitination
T87 Phosphorylation
S92 Phosphorylation
S97 Phosphorylation
S99 Phosphorylation
K102 Ubiquitination
S111 Phosphorylation
K150 Ubiquitination
S153 Phosphorylation
S156 Phosphorylation
S163 Phosphorylation
T165 Phosphorylation
K199 Ubiquitination
S207 Phosphorylation
S209 Phosphorylation
K212 Ubiquitination
Y234 Phosphorylation
S270 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
T275 Phosphorylation
S276 Phosphorylation
K278 Ubiquitination
T280 Phosphorylation
S289 Phosphorylation
T291 Phosphorylation
K297 Ubiquitination
T298 Phosphorylation
S300 Phosphorylation
K301 Acetylation
K301 Ubiquitination
K311 Ubiquitination
S316 Phosphorylation
S318 Phosphorylation
K323 Acetylation
K325 Ubiquitination
S330 Phosphorylation
S352 Phosphorylation
S354 Phosphorylation
T358 Phosphorylation
S364 Phosphorylation
S376 Phosphorylation
K397 Ubiquitination
K405 Ubiquitination
S410 Phosphorylation
S415 Phosphorylation
K416 Ubiquitination
S419 Phosphorylation
S424 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
S432 Phosphorylation
S437 Phosphorylation
S441 Phosphorylation
T442 Phosphorylation
S466 Phosphorylation
T501 Phosphorylation
S503 Phosphorylation
S536 Phosphorylation
T555 Phosphorylation
S567 Phosphorylation
K581 Ubiquitination
S591 Phosphorylation
S593 Phosphorylation
S641 Phosphorylation
S644 Phosphorylation
K648 Ubiquitination
S652 Phosphorylation
Y657 Phosphorylation
T659 Phosphorylation
K664 Ubiquitination
S665 Phosphorylation
S668 Phosphorylation
T682 Phosphorylation
S687 Phosphorylation
K701 Methylation
K701 Ubiquitination
S704 Phosphorylation
S706 Phosphorylation
S713 Phosphorylation
Y725 Phosphorylation
Y732 Phosphorylation
S737 Phosphorylation
S739 Phosphorylation
T742 Phosphorylation
T743 Phosphorylation
S746 Phosphorylation
S752 Phosphorylation
S759 Phosphorylation
K762 Ubiquitination
T764 Phosphorylation
S767 Phosphorylation
S770 Phosphorylation
T772 Phosphorylation
Y773 Phosphorylation
K774 Ubiquitination
Y775 Phosphorylation
T776 Phosphorylation
K785 Ubiquitination
S794 Phosphorylation
K797 Ubiquitination
S798 Phosphorylation
S803 Phosphorylation
S805 Phosphorylation
S806 Phosphorylation
K808 Sumoylation
K808 Ubiquitination
T828 Phosphorylation
S832 Phosphorylation
S840 Phosphorylation
T843 Phosphorylation
T864 Phosphorylation
S866 Phosphorylation
T867 Phosphorylation
S869 Phosphorylation
S883 Phosphorylation
T895 Phosphorylation
S902 Phosphorylation
S923 Phosphorylation
Y930 Phosphorylation
S944 Phosphorylation
K947 Ubiquitination
S948 Phosphorylation
R972 Methylation
S1040 Phosphorylation
S1053 Phosphorylation
S1055 Phosphorylation
S1060 Phosphorylation
S1063 Phosphorylation
S1115 Phosphorylation
S1118 Phosphorylation
S1121 Phosphorylation
S1131 Phosphorylation
T1132 Phosphorylation
S1134 Phosphorylation
T1140 Phosphorylation
S1152 Phosphorylation
T1156 Phosphorylation
T1158 Phosphorylation
S1161 Phosphorylation
T1163 Phosphorylation
S1165 Phosphorylation
T1168 Phosphorylation
S1174 Phosphorylation
S1175 Phosphorylation
K1200 Ubiquitination
S1203 Phosphorylation
K1212 Ubiquitination
K1227 Ubiquitination
S1247 Phosphorylation
K1250 Ubiquitination
K1255 Ubiquitination

Research Backgrounds

PTMs:

A 33 kDa peptide corresponding to the C-terminus of this protein is found in the testis and seems to be cleaved into 2 peptides of 14 kDa and 18 kDa found on the surface of mature sperm cells. This sperm surface antigen may be involved in some step of early cleavage of the fertilized oocyte.

Subcellular Location:

Cytoplasm.
Note: Located near the plasma membrane. Associated with actin filaments. May also exist as a membrane-bound form with extracellular regions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in pancreas and testis. Present in colon cancer cells (at protein level).

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