INVS Antibody - #DF12644
| Product: | INVS Antibody |
| Catalog: | DF12644 |
| Description: | Rabbit polyclonal antibody to INVS |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 97 kDa; 118kD(Calculated). |
| Uniprot: | Q9Y283 |
| RRID: | AB_2845606 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12644, RRID:AB_2845606.
Fold/Unfold
INV; Inversion of embryo turning homolog; inversion of embryonic turning; INVS; Nephrocystin 2; Nephrocystin-2; Nephrocystin2; nephronophthisis 2 (infantile); NPH2; NPHP2;
Immunogens
- Q9Y283 INVS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKSENLLFAGSSLASQVHAAAVNGDKGALQRLIVGNSALKDKEDQFGRTPLMYCVLADRLDCADALLKAGADVNKTDHSQRTALHLAAQKGNYRFMKLLLTRRANWMQKDLEEMTPLHLTTRHRSPKCLALLLKFMAPGEVDTQDKNKQTALHWSAYYNNPEHVKLLIKHDSNIGIPDVEGKIPLHWAANHKDPSAVHTVRCILDAAPTESLLNWQDYEGRTPLHFAVADGNVTVVDVLTSYESCNITSYDNLFRTPLHWAALLGHAQIVHLLLERNKSGTIPSDSQGATPLHYAAQSNFAETVKVFLKHPSVKDDSDLEGRTSFMWAAGKGSDDVLRTMLSLKSDIDINMADKYGGTALHAAALSGHVSTVKLLLENNAQVDATDVMKHTPLFRACEMGHKDVIQTLIKGGARVDLVDQDGHSLLHWAALGGNADVCQILIENKINPNVQDYAGRTPLQCAAYGGYINCMAVLMENNADPNIQDKEGRTALHWSCNNGYLDAIKLLLDFAAFPNQMENNEERYTPLDYALLGERHEVIQFMLEHGALSIAAIQDIAAFKIQAVYKGYKVRKAFRDRKNLLMKHEQLRKDAAAKKREEENKRKEAEQQKGRRSPDSCRPQALPCLPSTQDVPSRQSRAPSKQPPAGNVAQGPEPRDSRGSPGGSLGGALQKEQHVSSDLQGTNSRRPNETAREHSKGQSACVHFRPNEGSDGSRHPGVPSVEKSRGETAGDERCAKGKGFVKQPSCIRVAGPDEKGEDSRRAAASLPPHDSHWKPSRRHDTEPKAKCAPQKRRTQELRGGRCSPAGSSRPGSARGEAVHAGQNPPHHRTPRNKVTQAKLTGGLYSHLPQSTEELRSGARRLETSTLSEDFQVSKETDPAPGPLSGQSVNIDLLPVELRLQIIQRERRRKELFRKKNKAAAVIQRAWRSYQLRKHLSHLRHMKQLGAGDVDRWRQESTALLLQVWRKELELKFPQTTAVSKAPKSPSKGTSGTKSTKHSVLKQIYGCSHEGKIHHPTRSVKASSVLRLNSVSNLQCIHLLENSGRSKNFSYNLQSATQPKNKTKP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y283 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K41 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| R726 | Methylation | Uniprot | |
| R734 | Methylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| R799 | Methylation | Uniprot | |
| T864 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| T866 | Phosphorylation | Uniprot | |
| S980 | Phosphorylation | Uniprot | |
| K984 | Acetylation | Uniprot | |
| S985 | Phosphorylation | Uniprot | |
| K988 | Acetylation | Uniprot | |
| K1012 | Ubiquitination | Uniprot | |
| S1019 | Phosphorylation | Uniprot | |
| S1030 | Phosphorylation | Uniprot | |
| S1032 | Phosphorylation | Uniprot | |
| K1062 | Acetylation | Uniprot |
Research Backgrounds
Required for normal renal development and establishment of left-right axis. Probably acts as a molecular switch between different Wnt signaling pathways. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. Involved in the organization of apical junctions in kidney cells together with NPHP1, NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity).
May be ubiquitinated via its interaction with APC2.
Hydroxylated at Asn-75, most probably by HIF1AN.
Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle. Membrane>Peripheral membrane protein. Nucleus. Cell projection>Cilium.
Note: Associates with several components of the cytoskeleton including ciliary, random and polarized microtubules. During mitosis, it is recruited to mitotic spindle. Frequently membrane-associated, membrane localization is dependent upon cell-cell contacts and is redistributed when cell adhesion is disrupted after incubation of the cell monolayer with low-calcium/EGTA medium.
Widely expressed. Strongly expressed in the primary cilia of renal tubular cells.
Binds calmodulin via its IQ domains. Interacts with APC2. Interacts with alpha-, beta-, and gamma-catenin. Interacts with N-cadherin (CDH2). Interacts with microtubules (By similarity). Interacts with NPHP1. Interacts with DVL1, PRICKLE (PRICKLE1 or PRICKLE2) and Strabismus (VANGL1 or VANGL2). Interacts with IQCB1; the interaction likely requires additional interactors. Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme.
The D-box 1 (destruction box 1) mediates the interaction with APC2, and may act as a recognition signal for degradation via the ubiquitin-proteasome pathway.
Research Fields
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
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