IMPDH1 Antibody - #DF12642

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Product: IMPDH1 Antibody
Catalog: DF12642
Description: Rabbit polyclonal antibody to IMPDH1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 60 kDa, 70 kDa; 55kD(Calculated).
Uniprot: P20839
RRID: AB_2845604

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IMPDH1 Antibody detects endogenous levels of total IMPDH1.
RRID:
AB_2845604
Cite Format: Affinity Biosciences Cat# DF12642, RRID:AB_2845604.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IMDH1_HUMAN; IMP (inosine monophosphate) dehydrogenase 1; IMP dehydrogenase 1; IMPD 1; IMPD; IMPD1; IMPDH 1; IMPDH I; IMPDH-I; Impdh1; Inosine 5' monophosphate dehydrogenase 1; Inosine monophosphate dehydrogenase 1; Inosine-5''-monophosphate dehydrogenase 1; LCA11; RP10; sWSS2608;

Immunogens

Immunogen:
Uniprot:

P20839(IMDH1_HUMAN) >>Visit HPA database.

Gene(ID):
IMPDH1(3614)
Expression:
P20839 IMDH1_HUMAN:

IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

Sequence:
MADYLISGGTGYVPEDGLTAQQLFASADGLTYNDFLILPGFIDFIADEVDLTSALTRKITLKTPLISSPMDTVTEADMAIAMALMGGIGFIHHNCTPEFQANEVRKVKKFEQGFITDPVVLSPSHTVGDVLEAKMRHGFSGIPITETGTMGSKLVGIVTSRDIDFLAEKDHTTLLSEVMTPRIELVVAPAGVTLKEANEILQRSKKGKLPIVNDCDELVAIIARTDLKKNRDYPLASKDSQKQLLCGAAVGTREDDKYRLDLLTQAGVDVIVLDSSQGNSVYQIAMVHYIKQKYPHLQVIGGNVVTAAQAKNLIDAGVDGLRVGMGCGSICITQEVMACGRPQGTAVYKVAEYARRFGVPIIADGGIQTVGHVVKALALGASTVMMGSLLAATTEAPGEYFFSDGVRLKKYRGMGSLDAMEKSSSSQKRYFSEGDKVKIAQGVSGSIQDKGSIQKFVPYLIAGIQHGCQDIGARSLSVLRSMMYSGELKFEKRTMSAQIEGGVHGLHSYEKRLY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
89
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20839 As Substrate

Site PTM Type Enzyme
K109 Ubiquitination
S122 Phosphorylation
K134 Ubiquitination
K153 Ubiquitination
S160 Phosphorylation
K169 Ubiquitination
K195 Ubiquitination
R203 Methylation
K205 Ubiquitination
K206 Ubiquitination
K208 Ubiquitination
R231 Methylation
S237 Phosphorylation
K242 Acetylation
K242 Ubiquitination
T252 Phosphorylation
K293 Ubiquitination
T306 Phosphorylation
K311 Ubiquitination
R322 Methylation
Y400 Phosphorylation
K410 Ubiquitination
S416 Phosphorylation
K422 Ubiquitination
K428 Ubiquitination
Y430 Phosphorylation
S432 Phosphorylation
K436 Acetylation
K436 Ubiquitination
K438 Ubiquitination
K450 Ubiquitination

Research Backgrounds

Function:

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the IMPDH/GMPR family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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