Product: ICMT Antibody
Catalog: DF12639
Description: Rabbit polyclonal antibody to ICMT
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: O60725
RRID: AB_2845601

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
ICMT Antibody detects endogenous levels of total ICMT.
RRID:
AB_2845601
Cite Format: Affinity Biosciences Cat# DF12639, RRID:AB_2845601.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1700008E11Rik; C80758; fcmt; Gm13095; HSTE14; Icmt; ICMT_HUMAN; Isoprenylcysteine carboxylmethyltransferase; MGC118506; MGC39955; MGC95322; MST098; MSTP098; OTTMUSG00000010406; pcCMT; PCMT; PPMT; Prenylated protein carboxyl methyltransferase; Prenylcysteine carboxyl methyltransferase; Protein-S-isoprenylcysteine O-methyltransferase; RP23-421E12.4; STE14;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O60725 ICMT_HUMAN:

Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.

Sequence:
MAGCAARAPPGSEARLSLATFLLGASVLALPLLTRAGLQGRTGLALYVAGLNALLLLLYRPPRYQIAIRACFLGFVFGCGTLLSFSQSSWSHFGWYMCSLSLFHYSEYLVTAVNNPKSLSLDSFLLNHSLEYTVAALSSWLEFTLENIFWPELKQITWLSVTGLLMVVFGECLRKAAMFTAGSNFNHVVQNEKSDTHTLVTSGVYAWFRHPSYVGWFYWSIGTQVMLCNPICGVSYALTVWRFFRDRTEEEEISLIHFFGEEYLEYKKRVPTGLPFIKGVKVDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Rabbit
100
Zebrafish
88
Chicken
80
Xenopus
70
Sheep
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60725 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
K278 Ubiquitination

Research Backgrounds

Function:

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.

Family&Domains:

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.

Research Fields

· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.

Restrictive clause

 

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For Research Use Only.
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