GSTO2 Antibody - #DF12627

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Product: GSTO2 Antibody
Catalog: DF12627
Description: Rabbit polyclonal antibody to GSTO2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit
Mol.Wt.: 28 kDa; 28kD(Calculated).
Uniprot: Q9H4Y5
RRID: AB_2845589

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(86%), Bovine(83%), Sheep(83%), Rabbit(92%)
Clonality:
Polyclonal
Specificity:
GSTO2 Antibody detects endogenous levels of total GSTO2.
RRID:
AB_2845589
Cite Format: Affinity Biosciences Cat# DF12627, RRID:AB_2845589.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA127L20.1; glutathione dependent dehydroascorbate reductase; Glutathione S transferase like protein; glutathione S transferase omega 2 2; Glutathione S transferase omega 2; Glutathione S-transferase omega-2; GSTO 2 2; GSTO 2; GSTO-2; GSTO2; GSTO2_HUMAN; MMA(V) reductase; monomethylarsonic acid reductase; novel glutathione S transferase;

Immunogens

Immunogen:
Uniprot:

Q9H4Y5(GSTO2_HUMAN) >>Visit HPA database.

Gene(ID):
GSTO2(119391)
Expression:
Q9H4Y5 GSTO2_HUMAN:

Expressed in a range of tissues, including the liver, kidney, skeletal muscle and prostate. Strongest expression in the testis.

Sequence:
MSGDATRTLGKGSQPPGPVPEGLIRIYSMRFCPYSHRTRLVLKAKDIRHEVVNINLRNKPEWYYTKHPFGHIPVLETSQCQLIYESVIACEYLDDAYPGRKLFPYDPYERARQKMLLELFCKVPHLTKECLVALRCGRECTNLKAALRQEFSNLEEILEYQNTTFFGGTCISMIDYLLWPWFERLDVYGILDCVSHTPALRLWISAMKWDPTVCALLMDKSIFQGFLNLYFQNNPNAFDFGLC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Pig
92
Zebrafish
86
Bovine
83
Sheep
83
Xenopus
73
Horse
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H4Y5 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
Y27 Phosphorylation
S28 Phosphorylation
K59 Ubiquitination
K101 Ubiquitination
K128 Ubiquitination

Research Backgrounds

Function:

Exhibits glutathione-dependent thiol transferase activity. Has high dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA).

Tissue Specificity:

Expressed in a range of tissues, including the liver, kidney, skeletal muscle and prostate. Strongest expression in the testis.

Family&Domains:

Belongs to the GST superfamily. Omega family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). Pivotal Role of GSTO2 in Ferroptotic Neuronal Injury After Intracerebral Hemorrhage. Journal of molecular neuroscience : MN, 2024 (PubMed: 38386166) [IF=3.1]

Application: WB    Species: Mouse    Sample: HT22 cells

Fig. 8. GSEA against the obtained GO database in hemin-treated HT22 cells and the expression of Gsto2 was detected by western blot. A GSEA plots of the gene sets upon the GO database after hemin treatment. B String PPI analysis of the interaction network of Gsto2. C, D Expression of Gsto2 detected by western blotting. E Level of lipid ROS detected by flow cytometry

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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