GSPT2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from NIH/3T3 cells, using GSPT2 Antibody.

Western blot analysis of extracts from Hela, using GSPT2 Antibody.

Product:	GSPT2 Antibody
Catalog:	DF12622
Description:	Rabbit polyclonal antibody to GSPT2
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	69 kDa; 69kD(Calculated).
Uniprot:	Q8IYD1
RRID:	AB_2845584

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

GSPT2 Antibody detects endogenous levels of total GSPT2.

RRID:

AB_2845584
Cite Format: Affinity Biosciences Cat# DF12622, RRID:AB_2845584.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

eRF3b; ERF3B_HUMAN; Eukaryotic peptide chain release factor GTP-binding subunit ERF3B; Eukaryotic peptide chain release factor subunit 3b; G1 to S phase transition 2; G1 to S phase transition protein 2 homolog; Gspt2; GST2; peptide chain release factor 3;

Immunogens

Immunogen:

Uniprot:

Q8IYD1(ERF3B_HUMAN) >>Visit HPA database.

Gene(ID):

GSPT2(23708)

Expression:

Q8IYD1 ERF3B_HUMAN:

Highly expressed in IUCC stage II colorectal cancer (CRC).

Sequence:

Q8IYD1 ERF3B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDSGSSSSDSAPDCWDQVDMESPGSAPSGDGVSSAVAEAQREPLSSAFSRKLNVNAKPFVPNVHAAEFVPSFLRGPTQPPTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEPVVENGEVEMALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYKDMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILPGFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

PTMs - Q8IYD1 As Substrate

Q8IYD1

Site	PTM Type	Source
S71	Phosphorylation	Uniprot
K102	Acetylation	Uniprot
S113	Phosphorylation	Uniprot
S191	Phosphorylation	Uniprot
S196	Phosphorylation	Uniprot
K237	Acetylation	Uniprot
K237	Ubiquitination	Uniprot
Y238	Phosphorylation	Uniprot
T249	Phosphorylation	Uniprot
T258	Phosphorylation	Uniprot
K267	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
K316	Ubiquitination	Uniprot
T321	Phosphorylation	Uniprot
K325	Acetylation	Uniprot
K325	Methylation	Uniprot
K325	Ubiquitination	Uniprot
K337	Acetylation	Uniprot
K337	Ubiquitination	Uniprot
K369	Ubiquitination	Uniprot
K375	Acetylation	Uniprot
K376	Acetylation	Uniprot
S380	Phosphorylation	Uniprot
K382	Acetylation	Uniprot
T444	Phosphorylation	Uniprot
K577	Ubiquitination	Uniprot
K598	Acetylation	Uniprot
K598	Ubiquitination	Uniprot
T607	Phosphorylation	Uniprot
K613	Ubiquitination	Uniprot
K619	Acetylation	Uniprot
K619	Ubiquitination	Uniprot
K622	Acetylation	Uniprot
K622	Ubiquitination	Uniprot

Research Backgrounds

Q8IYD1_ERF3B_HUMAN

Function:

Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Highly expressed in IUCC stage II colorectal cancer (CRC).

Subunit Structure:

Interacts with ETF1 (By similarity). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with UPF1 and PABPC1.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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GSPT2 Antibody - #DF12622