Product: Glypican4 Antibody
Catalog: DF12619
Description: Rabbit polyclonal antibody to Glypican4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 62 kDa; 62kD(Calculated).
Uniprot: O75487
RRID: AB_2845581

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(82%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Glypican4 Antibody detects endogenous levels of total Glypican4.
RRID:
AB_2845581
Cite Format: Affinity Biosciences Cat# DF12619, RRID:AB_2845581.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dJ900E8.1 glypican 4; Glypican proteoglycan 4; Gpc4; GPC4_HUMAN; K Glypican; K-glypican; Kglypican; OTTHUMP00000024052; Secreted glypican 4; Secreted glypican-4; UNQ474/PRO937;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MARFGLPALLCTLAVLSAALLAAELKSKSCSEVRRLYVSKGFNKNDAPLHEINGDHLKICPQGSTCCSQEMEEKYSLQSKDDFKSVVSEQCNHLQAVFASRYKKFDEFFKELLENAEKSLNDMFVKTYGHLYMQNSELFKDLFVELKRYYVVGNVNLEEMLNDFWARLLERMFRLVNSQYHFTDEYLECVSKYTEQLKPFGDVPRKLKLQVTRAFVAARTFAQGLAVAGDVVSKVSVVNPTAQCTHALLKMIYCSHCRGLVTVKPCYNYCSNIMRGCLANQGDLDFEWNNFIDAMLMVAERLEGPFNIESVMDPIDVKISDAIMNMQDNSVQVSQKVFQGCGPPKPLPAGRISRSISESAFSARFRPHHPEERPTTAAGTSLDRLVTDVKEKLKQAKKFWSSLPSNVCNDERMAAGNGNEDDCWNGKGKSRYLFAVTGNGLANQGNNPEVQVDTSKPDILILRQIMALRVMTSKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCPSEFDYNATDHAGKSANEKADSAGVRPGAQAYLLTVFCILFLVMQREWR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
91
Sheep
91
Rabbit
82
Zebrafish
80
Xenopus
73
Chicken
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75487 As Substrate

Site PTM Type Enzyme
S39 Phosphorylation
Y186 Phosphorylation
S357 Phosphorylation
T375 O-Glycosylation
T376 O-Glycosylation
T472 Phosphorylation
S473 Phosphorylation

Research Backgrounds

Function:

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).

Subcellular Location:

Cell membrane>Lipid-anchor>Extracellular side.

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the glypican family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

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